Molecular Detection of Mycobacterium tuberculosis Complex by GenoType®MTBDRplus from Patients Attending Bingham University Teaching Hospital, Jos, Nigeria
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 9, Issue 6
Abstract
Aims: The study was to investigate the presence of M. tuberculosis and the resistance to rifampicin and isoniazid in AFB-positive sputa of patients attending Bingham University Teaching Hospital, Jos, Nigeria. Study Design: This was a prospective cross-sectional laboratory-based observational study. Place and Duration of Study: The study was carried out in Bingham University Teaching Hospital (BUTH), Jos North, Plateau state, Northern Nigeria from April, 2013 to December 2013. Methodology: A total of 90 AFB positive samples were collected from BUTH, Jos, and further screened for Mycobacterium tuberculosis using HAINS GenoType®MTBDRplus (HAIN Lifescience, Nehren, Germany). Results: Eighty three (92%) out of the 90 AFB smear positive samples were positive for M. tuberculosis by GenoType®MTBDRplus line probe assay while the remaining seven (8%) were negative. Mycobacterium tuberculosis resistance to either or both RIF and INH were detected in 46/83 (55%) total cases; with RIF 24%, INH 17% and multidrug resistance (Resistance to both INH and RIF) 14%. The commonly observed mutation types in this research were rpoB MUT2B and inhA MUT1 for RIF and INH respectively. Conclusion: The performance of GenoType®MTBDRplus to detect M. tuberculosis was excellent and the resistance detected was high in this region. Also, the overall level of resistance is noteworthy and suggestive of adverse public health implications with risk of increasing infection rates, on-going transmission, delayed therapy, increased mortality as well as development of secondary resistance known as extensively drug-resistant tuberculosis (XDR-TB). Prompt case detection and treatment strategy should be strictly adhered and to do this, rapid case detection molecular techniques such as HAINS GenoType®MTBDRplus is recommended.
Authors and Affiliations
Chisom Olachi Ukaegbu, Agatha Ani, Agwu Ulu Nnachi
Keywords
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- EP ID EP342389
- DOI 10.9734/BJPR/2016/22991
- Views 97
- Downloads 0
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