Molecular identification and phylogenetic analysis of Chlamydophila abortus isolated from sheep and goats
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2021, Vol 13, Issue 1
Abstract
Chlamydophila abortus is one of the major causes of pregnancy failure (abortion) in sheep and goats in many countries. In the present study, milk samples from sheep and goat herds of West Azerbaijan, Iran were examined for C. abortus using PCR and nucleotide sequencing. A total number of 360 milk samples were randomly collected from sheep (n=180) and goats (n=180) of three different regions of West Azerbaijan province during 2018. DNA was isolated from samples and the nested-PCR was employed targeting the 16S rRNA gene for detection of Chlamydia spp. The omp gene was amplified and sequenced for the characterization of detected C. abortus.The results showed that 8.61% (95% CI: 6.13%â11.96%) of the examined samples (11.67% sheep and 5.56% goat milk samples) were positive for C. abortus. The frequency of positive samples in the central region was significantly higher than in other regions. Positive samples for C. abortus from animals with a history of abortion were significantly higher than those without a history of abortion. Positive samples in autumn were significantly higher than the other seasons and also, in animals more than four years old were significantly higher than other age groups. Sheep infection was significantly higher than the goats. Phylogenetic analysis based on the helicase gene showed that two sequenced isolates clustered closely with the other C. abortus isolates reported in the GenBank. In conclusion, small ruminants in West Azerbaijan province were contaminated with C. abortus and they could shed this organism into the milk.
Authors and Affiliations
Fariba Taheri,Abdolghaffar Ownagh,Karim Mardani,
Keywords
Related Articles
- EP ID EP722385
- DOI -
- Views 64
- Downloads 1
Seroprevalence of Theileria equi and Babesia caballi infection in Turkoman breed horses in Iran
Equine babesiosis is a hemoprotozoan tickborne disease with worldwide distribution and caused by Theileria equi and Babesia caballi. This study was conducted to determine the seroprevalence of T. equi and B. caballi infe...
Effects of curcumin and its nano-micelle formulation on body weight, insulin resistance, adiponectin, and blood biochemical parameters of streptozotocin-induced diabetic rats
The effects of curcumin and its nano-micelle form on body weight, insulin resistance, adiponectin, and blood biochemical parameters of streptozotocin-induced diabetic rats were studied. Diabetes was induced in fifty male...
Structure of pancreas in Palam Dove (Streptoplia selegalensis)
The purpose of this study was to have a general view of pancreas in one species of pigeon.Some disorders affect avian pancreas.Pancreatitis and Pancreatic degeneration have been described in many infections and deficien...
Common Carp ( Cyprinus carpio) parasites diversity and prevalence in Erbil aquacultures: gills, skin and intestinal infections
This study was carried out to investigate the causative agents of the parasitic diseases affecting common carp (Cyprinus carpio) in aquacultures in the Erbil region. At first, all fish were visually examined, then, micro...
Seroepidemiology of Coxiella Burnetii in commercial dairy herds in northeast of Iran
Q fever is an important zoonotic disease caused by infection with Coxiella burnetii. Limited information is available concerning theseroprevalence of Coxiella burnetii in Iran.A serological survey was conducted to desc...