MOLECULAR IDENTIFICATION OF ASPERGILLUS FLAVUS AND ITS ABILITY FOR MALATHION BIODEGRADATION
Journal Title: Romanian Journal of Biology - Plant Biology - Year 2018, Vol 63, Issue 1
Abstract
Pesticides are considered a vital part in agriculture worldwide. In spite of the fact that they enhanced the quality and yield of the agricultural production however they do have certain serious effects on the environment. This study was carried out for isolation and molecular identification of microorganisms from water naturally contaminated with pesticides. Moreover, also to evaluate the ability of the identified isolate for growth in the presence of malathion. Furthermore, the ability of identified isolate for potential degradation of malathion residue in aqueous media was evaluated. In this study based on morphological and molecular data, the selected isolate was identified as A. Flavus with respect to its relation to the data from gene bank. Also the lowest nucleotide diversity value between the tested isolate and A. flavus, support the identification of selected isolate as A. flavus. The identified isolate has grown successfully in a media supplemented with malathion much faster than without it. Hundred percent of malathion initial concentration was degraded within 36 days of incubation with A. flavus. Bioremediation of malathion residues in water using A. flavus isolate are promising and considered first reported.
Authors and Affiliations
ALY DERBALAH, MOSTAFA SAAD ALLAH, ISMAEL A. KHATAB
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