Molecular Identification of Cholesterol Oxidase Enzyme-Producing Streptomyces Bacteria in Soil of Lorestan Province, Iran
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 11
Abstract
Bacterial Cholesterol oxidase (CHO) is a monomeric, bi-functional and Flavin adenine dinucleotide-linked enzyme that catalyzes oxidation and isomerization cholesterol in the presence of molecular oxygen. Recently, powerful pesticide properties of these enzymes have been discovered on pest and in agriculture was used as a new generation of biological pesticides in cotton plants. This study aimed to isolate and molecular identification of Streptomyces bacteria producing CHO from Lorestan soil to provide biological pesticide in future. A total of 100 samples were collected from garden and agricultural land soils of Lorestan province. First, samples were cultured in cholesterolcontaining medium as an alone carbon source. Identification of grown bacteria was performed using the morphological characteristics, biochemical and microbial tests. Then, the colorimetric and browning of culture medium confirmed the activity of CHO. Then, the activity of cholesterol oxides was assayed under various temperature and pH. Obtained strains were confirmed by amplification of 16s rRNA gene using PCR technique and sequencing. A total of three producing cholesterol oxidase bacterial samples were isolated. The results showed that obtained strains belong to Streptomyces. The activity of cholesterol oxidase enzyme was confirmed by the colorimetric, and browning of culture medium tests. Finally, the molecular identity of the bacterial species was detected by sequencing PCR products. The results showed that the bacterial species, Streptomyces, isolated from the soil is capable of producing cholesterol oxidase enzyme.
Authors and Affiliations
Hamed Esmaeil Lashgarian| Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran, Sareh Jahanbakhsh| Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran, Kiana Shahzamani| Dept. of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran Corresponding Email: shahzamani.k@lu.ac.ir
Keywords
Related Articles
- EP ID EP12223
- DOI -
- Views 315
- Downloads 18
Effect of Water Pollution on Blood Elements in the Human Population of Hail, KSA
The relationship between contaminated drinking water with trace elements and thyroid diseases hypertension, liver functions disorder and kidney functions disorder was studied in this research. The thyroid diseases hyper...
Comparing the Effect of Adding Ketamine and Neostigmine to Bupivacaine 0.25 % for Epidural Analgesia among Patients Candidated for Elective Femoral Fracture Surgery
Pain is a complex medical problem that its inadequate postoperative control has adverse effects on patients’ physiological, metabolic and mental status. Adding new supplements will lead to an increased duration of an...
HOMICIDE BY CERVICAL SPINAL CORD GUNSHOT INJURY WITH SHOTGUN FIRE PELLETS: CASE REPORT
This case present a rare forensic case of cervical spinal gunshot injury of a female by her husband, a professional hunter, during a family fight with a shotgun fire pellets. The gunshot destroyed completely the cervic...
Quality of life, general health status and related factors in women of polycystic ovary syndrome in Yazd (IRAN) 2014
polycystic ovary syndrome leads to augmentation of behavioral disorders among women and the affects their quality of life. The present study is being carried out in order to assess the quality of life and the level of g...
Education of Correct Insulin Injection Technique amongst Diabetic Patients: Outcome Study from Malaysia
Effect of insulin relies on correct dose and appropriateness of insulin injection technique. However, inappropriate technique is common among diabetes patients. The impact of educating proper technique in these patients...