Molecular mechanism of circRNA hsa_circ_0000290 regulating Zika virus replication: a preliminary study
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 4
Abstract
Objective To investigate the effect of transfusion-transmitted Zika virus (ZIKV) on the expression of circular RNA (hsa_circ_0000290) and the role of hsa_circ_0000290 in ZIKV replication. Methods A549 or U251 cells were infected with ZIKV, and the total RNA were extracted continuously for 1-4 days. The relative expression level of hsa_circ_0000290 was detected by RT-qPCR; hsa_circ_0000290 was produced by transcription of PDHX (pyruvate dehydrogenase complex component X). After knocking down the expression of hsa_circ_0000290 by transfection with siRNA, the effect on ZIKV and PDHX expression was detected by RT-qPCR and Western blot. The localization of hsa_circ_0000290 in cells was verified by cytoplasmic localization experiment. Results Compared with the control group, hsa_circ_0000290 was up-regulated in A549 and U251 cells after 1-4 days of ZIKV infection; hsa_circ_0000290 was mainly localized in the nucleus. Knockdown the expression level of hsa_circ_0000290 in cells can inhibit the replication of ZIKV RNA and the expression of ZIKV protein, and promote the expression of PDHX. Conclusion The expression level of hsa_circ_0000290 is significantly increased after ZIKV infection. Hsa_circ_0000290 is localized in the nucleus, and the specific knockdown of hsa_circ_0000290 expression level significantly inhibits ZIKV replication.
Authors and Affiliations
Lan KANG, Haiyan YE, Yike HUANG, Min XU, Limin CHEN, Xiaoqiong DUAN, Shilin LI
Keywords
Related Articles
- EP ID EP738523
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.04.003
- Views 32
- Downloads 0
Transfusion of a universal platelet product in hematologic patients for emergency
Objective To explore the clinical application of a universal platelet product for emergency, which was prepared by suspending O-type apheresis platelet concentrate in AB-type fresh frozen plasma, so as to improve the pla...
Correlation between heart rate variability and psychological evaluation before blood donation
Objective To investigate the correlation between heart rate variability (HRV) and the degree of nervousness before blood donation. Methods The psychological state of 253 blood donors before blood donation was assessed by...
Study on the protective effect of rapamycin-treated dendritic cells on transfusion-related acute lung injury after infection
Objective To investigate the viability of rapamycin-treated rapamycin-treated dendritic cells (DCs) in intervening transfusion-related acute lung injury (TRALI) after infection. Methods 1)The TRALI mouse model was induce...
Expert consensus on intraarticular injection of autologous apheresis platelet-rich plasma in the treatment of knee osteoarthritis
Knee osteoarthritis (KOA) is one of the most common musculoskeletal diseases. Platelet-rich plasma (PRP) has been widely used in the treatment of KOA. However, the effectiveness of KOA treatment has been affected due to...
Effect of ion-exchange chromatography on removal of fibronectin in von Willebrand factor
Objective To study the removal effect of fibronectin(Fn) from von willebrand factor(vWF) by ion-exchange chromatography through processing human coagulation factor Ⅷ chromatographic washing products, in order to select a...