Molecular methods for identification of monophasic Salmonella Typhimurium strains
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 4
Abstract
Two molecular biology methods were used to differentiate [i]Salmonella enterica[/i] 1,4,[5],12:i:- strains: “[i]Salmonella[/i] Check&Trace microarray” (CT) and multiplex PCR (mPCR). For 92 strains in CT result “[i]Salmonella[/i] 1,4,[5],12:i:-” were obtained. Those strains were confirmed in mPCR as monophasic[i] fljB[/i]-lack [i]Salmonella[/i] Typhimurium. For 17 strains, which in CT assay were recognized as [i]Salmonella[/i] Typhimurium, the same identification was obtained in mPCR. Reference [i]Salmonella[/i] strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as [i]Salmonella [/i]genovar, in mPCR – as [i]Salmonella[/i] O:4, H:i other than [i]Salmonella[/i] Typhimurium, the same like [i]Salmonella[/i] Farsta, recognized incorrectly in CT as [i]Salmonella [/i]Typhimurium.
Authors and Affiliations
Grzegorz Madajczak, Bożena Dera-Tomaszewska, Jolanta Szych, Anna Chróst, Monika Wasiak
Keywords
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