MOLECULAR PHYLOGENETIC ANALYSIS OF RARE SILENEAE SPECIES OF THE UKRAINIAN FLORA
Journal Title: Наукові доповіді НУБіП України - Year 2018, Vol 3, Issue 3
Abstract
The tribe Sileneae DC. (Caryophyllaceae) is estimated to contain 59 species in the Ukrainian flora, seven of which are listed in the Red Data Book of Ukraine (2009). Some of these taxa still do not have any registered sequences in the GenBank. The aim of the present study is to infer phylogenetic relationships of Ukrainian rare species within the Sileneae tribe from nucleotide variation in the nrDNA. ITS1-5.8S-ITS2 regions were sequenced for 11 taxa (12 sequences in total): Lychnis chalcedonica L., L. flos-cuculi L., Silene baccifera (L.) Roth, S. chlorantha (Willd.) Ehrh., S. cretacea Fisch. ex Spreng., S. frivaldszkyana Hampe (2 sequences), S. sytnikii Krytzka, Novosad et Protopopova, S. syreistschikowii P. Smirn., S. supina M. Bieb., S. vulgaris (Moench) Garcke, and S. zawadzkii Herbich. In addition to the obtained nrDNA fragments, 39 sequences of Sileneae were imported from GenBank. Agrostemma githago L. JN589107 was used as an outgroup. In total, 51 sequences were used to reconstruct the phylogeny using Bayesian inference. In the obtained phylogenetic tree, five major branches are observed: Atocion, Viscaria, Heliosperma, Lychnis, and Silene. Two rare species – Atocion lithuanicum (Zapał.) Tzvel. and A. hypanicum (Klok.) Tzvel. – are placed within Atocion clade. Endemic Heliosperma carpaticum (Zapał.) Klok. is sister to H. alpestre (Jacq.) Griseb. Viscaria and Lychnis clades do not contain any rare taxa. Four subclades are observed in Silene. The Carpathian endemic Silene zawadzkii (=Silenanthe zawadzkii (Herbich) Griseb. et Schenk, = Elisanthe zawadzkii (Herbich) Klok.) is sister to S. viscosa Pers. (=Carpophora viscosa (L.) Tzvelev, = Elisanthe viscosa (L.) Rupr.), but not to S. noctiflora (=Elisanthe noctiflora (L.) Willk.) and is nested within the second subclade of Silene. Other rare taxa are placed within the fourth subclade. Endemic to Eastern Crimea S. syreistschikowii forms a clade with closely related S. supina M. Bieb. Sister to them is S. cappadocica Boiss. & Heldr., which belongs to the same section Spergulifoliae Boiss. Widely distributed species in Southern Europe, but rare in Ukraine S. viridiflora L. has sister relationship to S. nutans L.; S. italica (L.) Pers. and S. nemoralis Waldst. & Kit. are also related to them. These four species belong to Silene sect. Siphonomorpha Otth. Ukrainian local endemic species S. sytnikii forms a single clade with Balkanian S. frivaldszkyana Hampe. Rare species S. cretacea is, surprisingly, sister to a group of taxa with small unisexual flowers from the sect. Otites (Adans.) Otth, but with low posterior probability.
Authors and Affiliations
V. O. Martyniuk, N. I. Karpenko, I. Yu. Kostikov
Keywords
Related Articles
- EP ID EP357853
- DOI -
- Views 83
- Downloads 0
SANITARY EVALUATION OF WASTE FROM THE PROCESSING OF SEA HYDROBINOTES USED FOR THE PRODUCTION OF FEES
The conducted researches established that among sea hydrobionts, which are used for the production of feeds, special sanitary safety is represented by mussels, which are used for the production of feed. They are seeded w...
MATHEMATICAL MODEL OF GENERALIZED COORDINATES OF A WORKING MEMBER OF A MULTI-AXIS MACHINE OF A PARALLEL STRUCTURE WITH AN EXCESS DRIVE
A promising task of modern machine-tool construction is the creation of high-performance multi-coordinate milling machines and machining centers with ultra-high speeds and accelerations, which is possible with the use of...
ETHOLOGY PIGS BY DIFFERENT CONDITIONS OF DETENTION
The results of studies on the ethological performance gilts and fattening pigs, depending on conditions. It was selected at 2 months of age 150 gilts, of which the three formed a group of 50 heads each. In the control gr...
DESIGN OF NON-LINEAR MATHEMATICAL MODEL FOR EVALUATION OF APPLICATION EFFICIENCY TO LANDSCAPE ZONES OF ZAKARPATTIA
To evaluate the application efficiency of agricultural lands through the perspective of soil fertility the degree of land use efficiency and the concept of agricultural sustainability as the practical implementation of s...
THE YIELD OF CORN DEPENDING ON SEED TREATMENT AND FOLIAR NUTRITION
The results of studies the effects of pre-sowing seed treatment with the preparation Polimiksobakteryn, as well as foliar nutrition with the microfertilizer Micro Mineralis (corn) and the growth biostimulator “Stympo” on...