Molecular typing of Malassezia furfur and Malassezia globosa isolated from Tinea versicolor patients, based on Total protein extract and DNA finger print profiles.
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 3
Abstract
The cellular proteins of the 40 studied isolates of Malassezia furfur were electrophoretically analyzed on SDS-PAGE. Analysis of the total cellular proteins from M. furfur isolates are grouped into two wide molecular weight range (11 - 112 kDa) . It is very interesting to notice that the band with molecular weight of 56 kDa may be specific to the type of tinea versicolor. The cluster analysis based on cellular proteins data of M. globosa separated the seven isolates into 2 groups according to the band with molecular weight 56 kDa, which is present in 6 cases (hyperpigmented) and absent in case ( hypopigmented ) lesions. The polymorphism observed from RAPD markers revealed a high degree of genetic diversity in M. furfur and M. globosa isolates collected from different patients having tinea versicolor infection. Only primer E (5'-TGCCGAGCTG-3') generated a RAPD PCR banding pattern which allowed the identification of 6 different amplification groups within M. furfur and M. globosa that could be used as diagnostic tool for different isolates of Malassezia..
Authors and Affiliations
Yehia A. -G. Mahmoud, Metwally A. Metwally, Hanan H. Mubarak, Nesma El Zewawy
Keywords
Related Articles
- EP ID EP654456
- DOI 10.24297/jab.v5i3.5459
- Views 181
- Downloads 0
Proton-Mr-Spectroscopy of the Breast: is it A Reliable Imaging Modality for Characterizing the Bi-Rads 3 And 4 Lesions Before the Biopsy?
Aim; Our aim is to evaluate the diagnostic performance of pre and post contrast proton MR spectroscopy (H-MRS) in patients with breast imaging-reporting data system (BI-RADS) 3 and 4 lesions. Materials and Meth...
FEEDING BEHAVIOUR AND SUCCESS OF CATTLE EGRET (BUBULCUS IBIS) INHABITING RURAL AND URBEN AREA OF LAXMANGARH CITY, RAJASTHAN, INDIA
.........
Effects of radiation leakage of microwave oven on male Swiss albino mice
Continuous microwave radiation leakage from microwave oven may be harmful for users who have high exposure to them. The aim of this work was to assess the effects of microwave leakage on blood, liver, kidney and testis o...
EFFECT OF DIETARY MONOSODIUM GLUTAMATE ON SOME ADIPOSE- DERIVED HORMONES IN ADULT MALE RATS
The current study was conducted to investigate the effect of dietary monosodium glutamate (MSG) on serum adiponectin, leptin, resistin hormone, lipid profile, body weights, food intake, and abdominal fat in adult m...
Interaction insect-microorganisms producers of ochratoxin A infesting cocoa (Theobroma cacao) bean stocks from Tonkpi region, Western in Co´te d Ivoire
The storage of cocoa beans in humid tropical area lead to their infection by insects and mold. The objectives of the current study were to make an inventory of insects and molds producing ochratoxin A and to highlight th...