Morphological, meristic characteristics and mtDNA analysis of Hampala Fish (Hampala macrolepidota Kuhl & Van Hasselt 1823) from ranau lake, Indonesia
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2014, Vol 5, Issue 2
Abstract
Hampala (Hampala macrolepidota) in Ranau Lake is locally known by three size-based names: small size as Kemencut, medium as Arongan and large as Sebarau. To know whether these local names are the same or different species, morphometric measurements, meristic counts and mtDNA analysis were carried out. Five individuals of each size, 157–172 mm total length for Kemencut, 264–295 mm for Arongan and 374–445 mm for Sebarau were taken. Morphometric measurements showed significant correlation (p > 0.01). The strongest correlation between body part and percent of total length was predorsal length (PL) with r = 0.980. Meristic character counts fit to the identification of Weber and Beaufort (1916). Number of scales along the lateral line and total number of pectoral fin were 27-28 and 15-16, respectively. Nucleotide base composition of H. macrolepidota did not show any variation in nucleotide situs. Intraspecific COI (Cytochrome Oxidase Subunit I) gene nucleotide-based phylogram of Hampala created a genetic relationship supported by boostrap value of 100%. No haplotypic variation was formed. All Hampala samples were included in H. macrolepidota (Kuhl & Van Hasselt 1823).
Authors and Affiliations
Safran Makmur, Diana Arfiati, Gatut Bintoro, Arning Wilujeng Ekawati
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