Morphological Neutrophils is most and lymphocyte least commonly effected hematological cells on peripheral blood smear after long duration steroids therapy for patients
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 4
Abstract
Aims & Objectives: Identify and exclude the misinterpretation of peripheral blood smears examination .Identify the steroid induced RBC & WBC morphological artifacts. Identify the steroids induced platelets related changes.Material & Methods: Blood was collected in a sterile EDTA containing tube and processed following our established laboratory protocol .A complete blood counting including HB%,PCV, Red cell indices ,platelet count and total white cell count and differential was done by Automated blood cell counter and peripheral blood smear examination then a sterile EDTA containing blood sample tube stored at room temperature. The all cell count indices including RBC,WBC count with differential along with morphological storage artifacts and platelet count with storages artifacts , was further confirmed by manual oil immersion smear study method. Peripheral smears study was done with field A and B stain and leishman stain.Conclusion: Steroid cause the various morphological changes in peripheral blood cells encountered on peripheral blood smear examination when smear prepared from prolong steroid therapy. Steroid cause WBC morphological and platelets related artifacts .These artifacts lead to various misinterpretation of peripheral blood smear examination so exclude them.
Authors and Affiliations
Dr. M. S. Koolwal
Keywords
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