Morphometric Assessment of Chronic Inflammatory Cells in Colonic Biopsies of Patients with Irritable Bowel Syndrome

Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 5

Abstract

ABSTRACT Introduction: Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder which has a complex pathophysiology including the role of inflammation being recently studied. Aim: To assess the role of mast cells and Intraepithelial Lymphocytes (IELs) in IBS patients by morphometry. Materials and Methods: In this study, 148 cases of IBS using the Rome III criteria and 28 controls were taken. IBS patients were divided into two subgroups of Post Infectious (PI) and Non Post Infectious (NPI) subgroups on the basis of past history of episodes of gastroenteritis. All of them underwent full length colonoscopy and biopsy from the descending colon. Histopathology of these two were compared with special attention to morphometric count of IELs by “pin hole” method and mast cells using toluidine blue highlighting. Results: Biopsies were unremarkable in 23(15.54%) patients, whereas 125 (84.46%) patients were diagnosed as Non Specific Colitis (NSC). The difference in IEL counts and mast cell counts were extremely significant (p<0.0001) in controls vs patients, controls vs PI-IBS patients and PI-IBS vs NPI-IBS subgroup whereas the difference was significant (p<0.001) in controls vs. NPI-IBS subgroup. Conclusion: The present study concluded that mast cells and IELs show a significant difference between IBS and non IBS cases, and could have an important role to play in the pathophysiology of IBS.

Authors and Affiliations

Anshul Singh, Anupriya Nautiyal, Shailesh R Patel, Vatsala Misra, SP Misra, Manisha Dwivedi

Keywords

Related Articles

Labour Epidural Analgesia: A Randomised Double Blind Comparative Study of 0.1% Levobupivacaine with Fentanyl vs. 0.1% Ropivacaine with Fentanyl

Introduction: Levobupivacaine and ropivacaine are suitable alternatives to bupivacaine for labour analgesia as they produce less motor blockade, decreased incidence of instrumental deliveries and less toxicity. Aim: To s...

Invasive Breast Carcinoma with Axillary Tuberculous Lymphadenitis

ABSTRACT The simultaneous occurrence of breast cancer and tuberculosis is rare. As there are no pathognomic clinical signs and symptoms, it can create problem in diagnosis and treatment of both diseases. A 50-year-old fe...

Effect of Yoga on Nonlinear Dynamics of Heart Rate in Prediabetic Subjects

ABSTRACT Introduction: Pre-diabetes represents the initial stage of type 2 diabetic disease developments. Prediabetes disease progression to overt type 2 diabetes occurs when pancreatic beta cells cannot produce insulin...

Gender Linked Metric Analysis of Portal Vein: A Sonographic Appraisal

Introduction: Portal hypertension is one of the most mystifying and disconcerting abdominal ailment. Ultrasonography (USG) is an effective diagnostic tool for its prompt management. Knowledge of normal calibre of portal...

Download PDF file
  • EP ID EP586010
  • DOI 10.7860/JCDR/2019/40569.12852
  • Views 101
  • Downloads 0

How To Cite

Anshul Singh, Anupriya Nautiyal, Shailesh R Patel, Vatsala Misra, SP Misra, Manisha Dwivedi (2019). Morphometric Assessment of Chronic Inflammatory Cells in Colonic Biopsies of Patients with Irritable Bowel Syndrome. Journal of Clinical and Diagnostic Research, 13(5), 6-9. https://europub.co.uk/articles/-A-586010