Mother-Child Pair Transmission of Streptococcus Mutans; PCR Based Study in Urban Population of Pakistan
Journal Title: Saudi Journal of Pathology and Microbiology - Year 2016, Vol 1, Issue 1
Abstract
Abstract: The aim of this study was to determine vertical transmission of Sterptococcusmutans(S. mutans) genotypes in mother-child pair. The present study was conducted on 60 mother-child pair saliva samples having at least three carious teeth. All enrolled preschool children were divided into two age groups Group-1 (1-3years) and Group-2 (4-5 years). Stimulated saliva samples were collected from all enrolled subjects. After isolation of S. mutans DNA, Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) and genotyping methods (with primers for the glucosyltransferase gene) were used to identify the matching and non-matching patterns of S. mutans genotypes transmitted from mother′s to their child. Statistical analysis showed 75% matching genotypes were found in Group-1 mother-child pair and 65 % was reported in Group-2 mother-child pair suggesting the presence of vertically transmitted strains of S. mutans higher in Group-1. Keywords: S. mutans, Dental caries, Vertical transmission, Arbitrarily Primed Polymerase Chain Reaction (AP-PCR).
Authors and Affiliations
Mariyah Javed, Sidra Butt, Sara Ijaz, Faiza Awais, Rabia Asad, Arsalan Wahid, Ayyaz Ali Khan, Saima Chaudhry, Muhammad Idrees Khan, MateenIzhar .
Keywords
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