Mucosal delivery of bacterial antigens and CpG oligonucleotides formulated in biphasic lipid vesicles in pigs
Journal Title: The AAPS Journal - Year 2005, Vol 7, Issue 3
Abstract
The ineffectiveness of simple delivery of soluble antigens to mucosal membranes for immunization has stimulated extensive studies of strategies for appropriate delivery systems and adjuvants. Biphasic lipid vesicles are formulations suitable for the delivery of proteins, peptides, and oligo/polynucleotides. The purpose of these studies was to investigate the ability of biphasic lipid vesicles (as vaccinetargeting adjuvants) containing a bacterial antigen and unmethylated oligonucleotides containing CGdinucleotides-CpG motifs (CpG ODNs) to induce systemic and mucosal immune responses in pigs. Results showed that while the protein, either alone or with CpG ODNs, did not induce mucosal immune responses, administration of antigen and CpG ODNs in biphasic lipid vesicles resulted in induction of boty systemic and local antibody responses after immunization using a combined mucosal/systemic approach.
Authors and Affiliations
Valeria Alcon, Maria Baca-Estrada, Marco Vega-Lopez, Philip Willson, Lorne A. Babiuk, Praveen Kumar, Rolf Hecker, Marianna Foldvari
Keywords
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- EP ID EP681890
- DOI 10.1208/aapsj070357
- Views 69
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