MULTIPLEX PCR FOR RAPID DETECTION OF EXONAL DELETIONS IN PATIENTS OF DUCHENNE MUSCULAR DYSTROPHY

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MULTIPLEX PCR FOR RAPID DETECTION OF EXONAL DELETIONS IN PATIENTS OF DUCHENNE MUSCULAR DYSTROPHY

Journal Title: Indian Journal of Clinical Biochemistry - Year 2006, Vol 21, Issue 1

Abstract

Duchenne muscular dystrophy (DMD) is the most common X-linked disorder in children affecting 1 in 3500 males. Since, as of now, we have no treatment for DMD, carrier detection and prenatal diagnosis is the most important preventive strategy. Multiplex PCR helps in rapid detection of hot spot exonal deletions (positive in 65% of cases ) as many exons can be identified in a single run. 10 children with characterstic clinical features of DMD and chorionic villus samples of 10 antenatal patients with positive family history were studied. We identified a deletion mutation in exon 49 of the dystrophin gene in a 4 yr old boy referred with signs and symptoms suggestive of DMD using primers for exons 45, 48, 49, 43, 44, 19, 3, 8, 13 and muscle promoter,subjected to multiplex polymerase chain reaction (PCR) and agarose/Nu-Sieve gel electrophoresis. These genetic methods aid in prenatal diagnosis of DMD as well as confirmation of diagnosis in children with signs and symptoms suggestive of the disease.

Authors and Affiliations

Ritu Singh , Vijjaya and Madhulika Kabra

Keywords

EP ID EP118396
DOI -
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