Multiplication of Ipomoea batatas clone 'INIVITB2-2005' in Temporary Immersion System
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 2
Abstract
Use of temporary immersion system (TIS) have been not reported for in vitro culture of Ipomoea batatas . This technique would help to increase the number of plants needed for in vitro mass propagation of clone ‘INIVITB2- 2005’ with great productive potential. The aim of this paper was to multiply this clone in TIS of 200 ml capacity. Influence of immersion time, frequency of immersion, volume of culture medium per explant and subculture time on growth and multiplication of in vitro plants in TIS was determined. Length of explants (cm) was measured. Number of internodes per explant and number of active leaves was quantified . Fresh mass (g) of in vitro plants and multiplication coefficient was also determined . The best results were achieved with an immersion frequency of 10 minutes every three hours, using 15 ml of culture medium per explant and subcultures every 30 days. A multiplication coefficient of 8.01was reached.
Authors and Affiliations
Milagros Basail Pérez, Víctor Medero Vega, Manuel Cabrera Jova, Arletys Santos Pino, Marlenys Torres Delgado, Eneida Otero Gálvez, Alexi Ortega Ortiz, Jorge López Torres, Aymé Rayas Cabrera, Yoel Beovides García, Eriker Paz Chávez
Keywords
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