Mutational Analysis of DNASE I Gene in Diabetic Patients
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2013, Vol 3, Issue 3
Abstract
The main aim is to analyze the mutations of DNASE I gene in diabetic patients.A total of 120 diabetes patients and 120 controls were sampled. The total number of male diabetic patients included in the study was 79 (66%) while female patients were 41 (34%) in number. Exon 8 of the DNASE I gene was amplified by using thermocycler. The possible band of interest was located at 165 base pairs. Two samples showed similar missense mutations at 127th position of exon 8 which replaced amino acid Arginine (Arg) to Glutamine (Gln). All controls showed no mutations. The association of diabetes with different levels of blood pressure and body mass index (BMI) were found to be significant.
Authors and Affiliations
Nageen Hussain, Hateem Zafar Kayani
Keywords
Related Articles
- EP ID EP653842
- DOI 10.24297/jbt.v3i3.5025
- Views 91
- Downloads 0
EVALUATION OF DIFFERENT PRE-SETTING CONDITIONS IN AIRLIFT BIOREACTOR TO DETERMINE THE RESPIRATORY KINETICS OF FUNGI
in culture media and, therefore, used to obtain large volumes of fungal tissue to be used as inoculum. The implementation of controlled mycorrhization programs is dependent upon the production of commercially large volum...
Association Between ACE I/D Gene Polymorphism and Hypertension in Smokers
The main aim is to study the association of ACE I/D polymorphism with healthy smokers. A total of 49 smokers and 49 healthy controls were enrolled in this study. Intron 16 of the ACE gene was amplified by using nested PC...
1,3-PROPANEDIOL: STATISTICAL OPTIMIZATION OF MEDIUM TO IMPROVE PRODUCTION BY Clostridium beijerinckii DSM 791
Biodiesel is a promising alternative biofuel, and as its production increases, so does production of the principal co-product, glycerol. The present study is aimed at maximizing the glycerol consumption and 1,3-propanedi...
The Pathways Involved in TLQP-62 Mediated Biological Functions
VGF is neurosecretory protein, belongs to the extendedgranin family of proteins, originallyrecognized as a nerve growth factor (NGF) inducible gene product. It is selectively synthesized mostly in neuronal and neuroendoc...
Induction of callus and somatic embryogenesis from explants of Parkiabiglobosa (Jacq.)Benth and ParkiaroxburghiiG.Don.
Legumes are very important species including herbs, shrubs and trees, they are cultivated worldwide for different economic uses.Parkiais genus belongs to the Legumefamily, Fabaceae. The most common known species are P. b...