NASAL CARRIAGE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AMONG MEDICAL STAFF AT OMDURMAN TEACHING HOSPITAL
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2015, Vol 4, Issue 1
Abstract
Background: methicillin-resistant Staphylococcus aureus( MRSA) is a strain of Staphylococcus aureus which is resistant to methicillin and other antibiotics. Staphylococcus aureus is an organism that colonizes mainly in the nose, skin folds, hairline, perineum and navel in human. A patient becomes clinically infected if the organism invades the skin or deeper tissues and multiplies, other cases may remain subclinical. MRSA is prevalent in health care personal because their vulnerability to infection is higher than general population. Methods: This was descriptive cross sectional study in which atotal of 200 nasal swabs were collected from medical staff, out of which 102 were from the nursing staff , 78were from clinical Staff and 20 from lab technician Sterile cotton swabs moistened with sterile saline were used for sample collection. Swabs were cultured on mannitol salt agar, incubated at 37OCfor 24 hrs. Staphylococcus aureus was identified by morphology, Gram stain, catalase test, coagulase test, and mannitol salt agar fermentation. Methicillin resistance was detected by Kirby Bauer disk diffusion method. All methicillin-resistant isolates were examined for the existence of the mecA gene by conventional PCR. Results: Of the 200 samples screened 33 (16.5%) isolates of Staphylococcus aureus were identified, out of which 10 (30.3%) were methicillin resistant Staphylococcus aureus(MRSA) while 23 (69.7%) were methicillin sensitive Staphylococcus aureus(MSSA). The overall carriage rate of methicillin resistant Staphylococcus aureus in our study was the highest rate being seen among the nursing staff (51%) and clinical staff (39%) and laboratory technician (10%) were slightly less than nursing staff. Conclusion: Our study revealed that nursing staff were the potential colonisers of methicillin resistant Staphylococcus aureus when compared to clinical staff. In the present study about 5% of medical staff in Omdurman teaching hospital had methicillin resistant Staphylococcus aureus.
Authors and Affiliations
Hajir Mutasum Mohamed salh| M.Sc Student Microbiology Department of Faculty of Medical Laboratory Sciences, Al Neelain University, Sudan, Wafa Ibrahim Elhag| Association Professor- Microbiology Department, Faculty of Medical Laboratory Sciences, Al-Neelain University, Khalid Abd Alah Enan and Osama Mohammed M. Khair| Department of Virology, Central laboratory Khartoum, Sudan
Keywords
Related Articles
- EP ID EP3507
- DOI -
- Views 347
- Downloads 17
QUANTIFICATION OF HBV DNA AND ITS IMPACT ON VIRAL LOAD MONITORING
HBV is a causative agent for chronic hepatitis, and can cause liver cirrhosis and hepatocellular carcinoma. Chronic carriers are at high risk of long term complications of infection, including chronic hepatitis, cirrhos...
COMPARISON BETWEEN COBAS E411 AND ICT IN DIAGNOSIS OF HEPATITIS B VIRUS
Background: Hepatitis B is one of the world’s most common and serious infectious diseases. It is estimated that more than one third of the world’s population has been infected with hepatitis B virus Differentiation betwe...
Synthesis and spectrophotometric study of complexation of Cu (II) with 2-[Phenyl – (Phenyl Hydrazino) – Methyl] Phenyl Amine in Acetonitrile Solution
With respect to specious application of phenyl hydrazine and its derivative in organic Chemistry, Pharmacy and chemical industry. On its toxicity and biological effects, the 2-[Phenyl – (Phenyl Hydrazino) – Methyl] Pheny...
Clinical Indications of Hematopoietic Stem Cell Transplantation: A Clinical Review
Hematopoietic stem cells (HSCs) are multipotent, self-renewing progenitor cells that develop from mesodermal hemangioblast cells. All differentiated blood cells from the lymphoid and myeloid lineages arise from HSCs. H...
DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS OF INDOMETHACIN IN RABBIT BLOOD PLASMA BY RP-HPLC TECHNIQUE
The objective of the present work involved the development of simple, rapid, sensitive and cost effective RP-HPLC method for the estimation and quantification of indomethacin in rabbit plasma to evaluate their linearity...