Native antigen of Aspergillus fumigatus with useful for immunodiagnosis of aspergilloma

Apply

Native antigen of Aspergillus fumigatus with useful for immunodiagnosis of aspergilloma

Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2009, Vol 26, Issue 2

Abstract

In order to evaluate the usefulness of native antigens of autochthonous strains of Aspergillus fumigatus for immunodiagnosis of aspergilloma, We was conducted a study using two strains of this fungus, isolated from patients with aspergilloma (533 and 554), which were confronted with commercial control serum of A. fumigatus, A. flavus, A. niger, Candida,Coccidioides, Histoplasma and Paracoccidioides by immunodiffusion test, moreover, we evaluated 28 serum samples from patients with suspected aspergilloma. We also performed the characterization of the protein components of native antigens using the technique of SDS-PAGE and were confronted with ten serum samples from patients with aspergilloma, paracoccidioidomycosis, histoplasmosis, C-reactive protein and hydatidosis, also healthy person serum by immunoblot. Good agreement (kappa 0.92) was found between antigens 533 and 554 and somewhat less than those with commercial antigen A. fumigatus (kappa 0.73 and 0.81 for the 533 and 554, respectively). The band of 97 kDa reacted only with sera from patients with aspergilloma being immunodominant.

Authors and Affiliations

José Casquero, Flor Urcia, Elizabeth Sánchez

Keywords

Vertical distribution of the mosquito Haemagogus jantinomys (Dyar) (Diptera: Culicidae) in forests of the Peruvian Amazon.

[i][b]Objectives: [/b][/i]Our goals were to determine if the abundance of the mosquito [i]Haemagogus janthinomys[/i] is correlated with environmental variables, and if it differs between the canopy and understory in thre...

EP ID EP144663
DOI -
Views 80
Downloads 0