Natural Dye For Staining Atrocytes and Neurons
Journal Title: Journal of Neurological Sciences-Turkish - Year 2006, Vol 23, Issue 3
Abstract
Background: Natural dyes have been used for staining carpet, wool, silk, and cotton. Also, natural dyes have been used for histological staining purposes. As an example, tough, leathery resembling skin of pomegranate (Punica Granatum L.) fruit has strong staining activity. Aim of this study was to investigate a new staining method using pomegranate dye for histological purposes in the nervous system. Material and Methods: Whole brain of adult Wistar rats (12-13 week old) were removed, immersed in formaldehyde fixative, and embedded in paraffin. Sections, 7-10 micrometer from brain cortex, hippocampus and cerebellum, were collected, and then stained by the new method (dye extracted form pomegranate) and hematoxyllin & eosin staining method for control. Results: Analysis of sections stained by pomegranate dye have shown astrocytes of brain in yellow, astrocytes of hippocampus in deeply yellow, neurons of hippocampus and purkinje cells of cerebellum in pale red, granular cells of cerebellum, elastic fibers, and red blood cells in yellow. Conclusion: By using the dye extracted from skin of pomegranate, pathologists can make differentiation between neuron and astrocytes. This dye method can be an alternative to cost and time consuming current chemical staining methods in neuropathology.
Authors and Affiliations
Anneh GHARRAVI, Mohammad GOLALIPOUR, Rostam GHORBANI, Mozaffar KHAZAEI
Keywords
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