Advanced glycation end products in the plasma and renal tissues in experimental model of unilateral renal artery stenosis
Journal Title: Progress in Health Sciences - Year 2012, Vol 2, Issue 2
Abstract
Purpose: To evaluate whether advanced glycation end products (AGE) levels are increased in the plasma and renal tissues of rats after unilateral renal artery stenosis (RAS). Materials and methods: AGE levels were measured using commercially available ELISA kit in plasma and renal tissue samples obtained from 16 rats with experimental induced RAS for 3 and 28 days and from 6 respective sham-operated control rats. We also analyzed by HPLC the concentration of 4-hydroxynonenal (4-HNE), a known inducer of AGE formation and accumulation. Results: Plasma concentrations of 4-HNE and AGE were significantly increased (p<0.05) after 28 days of RAS. At this time point, the concentration of AGE was markedly increased in the clipped atrophic kidney (by about 10-fold; p<0.05), but it was unchanged in the contralateral kidney of the same rats. No differences in plasma and renal AGE levels were detected at day 3 of RAS. Sham operation did not affect the levels of AGE and 4-HNE at each time point. Conclusions: Increased accumulation of AGE both in the plasma and in the ischemic atrophic kidney suggest that AGE levels can be used as a reliable biomarker for monitoring the development of ischemic nephropathy caused by renal artery stenosis.
Authors and Affiliations
J. Brańska, Kruszewski, A. Tarasik
Keywords
Related Articles
- EP ID EP158648
- DOI -
- Views 76
- Downloads 0
Bystander cardiopulmonary resuscitation: Equipping communities to save lives
Majority of the occurrences of cardiac arrest happen outside the hospital and are associated with poor survival due to delay in recognition and provision of early cardiopulmonary resuscitation (CPR). Bystanders are often...
Lipodystrophy as dietary and cosmetic problem
<b>Introduction:</b> Lipodystrophy is a skin composition disorder. One of the kinds of lipodystrophy is cellulite. Differences in fat tissue composition between woman and man are the cause that lipodystrophy affects wome...
Body image and maternal fetal attachment
Introduction: Pregnancy is the time of the most dynamic and visible changes in a woman's body. Some researchers have shown that woman's ability to adapt to changes in her body may affect the attitude towards her baby....
The meaning of the nursing in Byzantium
History reminds us that the care in Byzantium constitutes a unique example for all of human history and civilization. The Byzantine period is specifically of interest for its approach to social welfare and organized care...
Role of a Balint group in hospice practice
Michael Balint was a Hungarian physician and psychoanalyst, and student of Sandor Ferencz, who applied in his medical practice elements of psychoanalysis. Balint argued that the role of the doctor or therapist r...