Assessment of genetic distance among wheat genotypes through RAPD markers
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2012, Vol 4, Issue 1
Abstract
PCR technology has made it possible to find out genetic diversity in crop plants. The present research work was carried out to determine the percentage of genetic distance among seven wheat genotypes by random amplified polymorphic DNA markers based on PCR technique. A total of 588 DNA fragments was obtained through 20 decamer primers with an average of 4.2 bands per primer. Out of 588, 189 (32%) DNA fragments showed polymorphism between seven wheat genotypes, while rest of DNA fragments was monomorphic. The genetic similarity of these genotypes was high ranging from 34.29 % to 71.43 %. The genotypes Iqbal-2000 and Uqab-2000 had the greatest similarity (71.43 %), while the lowest genetic similarity was found between the genotypes MH-97 and Uqab-2000. These results indicated that RAPD technique is useful to estimate the extent of genetic diversity in wheat as well as other crop species, these results can help to select such parental genotypes having wide differences compared to other existing genotypes for contributing high grain yield, wider adaptability and other related traits in wheat plant.
Authors and Affiliations
Shahzadi Mahpara, Jehanzeb Farooq, Zulfiqar Ali, I. Petrescu-Mag, Fida Hussain
Keywords
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