Assessment of genetic distance among wheat genotypes through RAPD markers
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2012, Vol 4, Issue 1
Abstract
PCR technology has made it possible to find out genetic diversity in crop plants. The present research work was carried out to determine the percentage of genetic distance among seven wheat genotypes by random amplified polymorphic DNA markers based on PCR technique. A total of 588 DNA fragments was obtained through 20 decamer primers with an average of 4.2 bands per primer. Out of 588, 189 (32%) DNA fragments showed polymorphism between seven wheat genotypes, while rest of DNA fragments was monomorphic. The genetic similarity of these genotypes was high ranging from 34.29 % to 71.43 %. The genotypes Iqbal-2000 and Uqab-2000 had the greatest similarity (71.43 %), while the lowest genetic similarity was found between the genotypes MH-97 and Uqab-2000. These results indicated that RAPD technique is useful to estimate the extent of genetic diversity in wheat as well as other crop species, these results can help to select such parental genotypes having wide differences compared to other existing genotypes for contributing high grain yield, wider adaptability and other related traits in wheat plant.
Authors and Affiliations
Shahzadi Mahpara, Jehanzeb Farooq, Zulfiqar Ali, I. Petrescu-Mag, Fida Hussain
Keywords
Related Articles
- EP ID EP156564
- DOI -
- Views 101
- Downloads 0
Effect of livelihood activities on food security among farmers in Oyo East Local Government Area of Oyo State, Nigeria
This study examined different livelihood activities and food security situations of the Nigerian’s major farm households considering rural farmers in Oyo East Local Government Area of Oyo State as case study. The study m...
Assessment of genetic distance among wheat genotypes through RAPD markers
PCR technology has made it possible to find out genetic diversity in crop plants. The present research work was carried out to determine the percentage of genetic distance among seven wheat genotypes by random amplified...
The impact of inaccessible health care services on agricultural production by rural farmers in Umuahia North Local Government Area, Abia state, Nigeri
Millions of people in the rural area suffer greatly because medical care is not readily available and is poor in quality. This study determined the households’ inaccessibility to health care services and agricultural pro...
Leaf petiole mineral and fruit heavy metals content of different grape cultivars grown under arid environments and irrigated with treated domestic wastewate
This study was carried out during 2008 and 2009 years at the Research and Agricultural Experimental Station, King Saud University, Saudi Arabia, in order to study the comparative effect of some grapevine cultivars grown...
The influence of climatic conditions on the main vine diseases in terms of chemicals application
This paper presents research results on the influence of climatic conditions on the occurrence and manifestation of main vine diseases. In the Blaj wine center the main grapes diseasesare blight (Plasmopara viticola), mi...