Assessment of genetic distance among wheat genotypes through RAPD markers
Journal Title: Advances in Agriculture & Botanics - International Journal of the Bioflux Society - Year 2012, Vol 4, Issue 1
Abstract
PCR technology has made it possible to find out genetic diversity in crop plants. The present research work was carried out to determine the percentage of genetic distance among seven wheat genotypes by random amplified polymorphic DNA markers based on PCR technique. A total of 588 DNA fragments was obtained through 20 decamer primers with an average of 4.2 bands per primer. Out of 588, 189 (32%) DNA fragments showed polymorphism between seven wheat genotypes, while rest of DNA fragments was monomorphic. The genetic similarity of these genotypes was high ranging from 34.29 % to 71.43 %. The genotypes Iqbal-2000 and Uqab-2000 had the greatest similarity (71.43 %), while the lowest genetic similarity was found between the genotypes MH-97 and Uqab-2000. These results indicated that RAPD technique is useful to estimate the extent of genetic diversity in wheat as well as other crop species, these results can help to select such parental genotypes having wide differences compared to other existing genotypes for contributing high grain yield, wider adaptability and other related traits in wheat plant.
Authors and Affiliations
Shahzadi Mahpara, Jehanzeb Farooq, Zulfiqar Ali, I. Petrescu-Mag, Fida Hussain
Keywords
Related Articles
- EP ID EP156564
- DOI -
- Views 99
- Downloads 0
The compared analysis of the agricultural legislation from the European integration<br /> perspective. The Iberic and Northern models.
The paper - The compared analysis of the agricultural legislation from the European integration perspective. The Iberic and Northern models - represents a short summary of the PhD thesis with the same title, defended in...
Contributions to the paludal vegetation study from the Vaslui river basin.
The paper presents four paludal associations in the Vaslui river basin: Caricetum elatae Koch 1926, Caricetum vulpinae Soó 1927, Bolboschoenetum maritimi Eggler 1933 and Schoenoplectetum tabernaemontani Soó 1947, associa...
Macrofungi from „Tinovul de la Româneşti” peat bog (Dornelor Depression, Romania).
„Tinovul de la Româneşti” is a forested peat bog, with an area of 20 ha, located in Dornelor Depression (Eastern Carpathians) within the limits of Coşna commune, Suceava County, Romania. The mycological observations carr...
Studies on genetic variability for yield components in rice (Oryza sativa L.)
The present experiment comprised with 3 CMS lines (viz IR58025A, NMS4A and PMS10A) and 20 diverse genotypes were crossed in a line x tester mating design to identify suitable parents for heterosis breeding. The anal...
Effects of seed hardening on growth and yield of chickpea ( Cicer arietinum L. )
A field experiment was conducted to study the efect of seed hardening with various chemicals on morpho-physiological traits and yield in chickpea (Cicer arietinum L.) during Rabi 2007, under rain fed conditions...