Assessment of prevalence of HPV DNA in colorectal adenocarcinoma in patients from the Podlasie region
Journal Title: Progress in Health Sciences - Year 2012, Vol 2, Issue 2
Abstract
Purpose: The aim of the study was to assess the prevalence of human papillomavirus (HPV) DNA in colorectal adenocarcinoma in patients from the Podlasie region undergoing surgery for the tumor. Materials and methods: We examined 40 solid colorectal tumors taken during surgical treatment at the 2nd Department of General and Gastrointestinal Surgery, Medical University of Białystok. HPV was detected by means of polymerase chain reaction (PCR) and in situ hybridization (ISH). The tests were performed on formalin-fixed, paraffin-embedded tumor tissue. Two pairs of primers were used for the detection of HPV DNA by PCR. Pair pU-1M/pU-2R enables detection and identification of high-risk HPV (HPV 16, 18, 31, 33, 35, 52b, 58), while pair pU-31B/pU-2R enables detection and identification of low-risk HPV (HPV 6, 11). The ISH was performed with the use of biotin-labelled dsDNA probes, using Wide Spectrum HPV DNA Probe Cocktail Biotinylated kit, DAKO Cyto-mation. Results: HPV DNA was found in 21 (52.5%) of the examined colorectal tumors. The PCR revealed the presence of viral DNA in 19 (47.5%) tumors. The ISH revealed the presence of HPV DNA in 16 (40%) of the examined tumors. Conclusion: The findings of this study correlate with similar results conducted by other research groups. However, this is the first study of colorectal tumor samples taken from patients of the Podlasie region. Therefore, the association between environmental factors, HPV infection, and tumor stage should also be verified in a larger study population. Further studies confirming the presence of HPV DNA in colorectal tumor tissue in populations from different regions of Poland are needed.
Authors and Affiliations
A. Miąsko, M. Chomczyk, J. Pancewicz–Wojtkiewicz, Kędra, B. Szynaka, L. Chyczewski
Keywords
Related Articles
- EP ID EP116990
- DOI -
- Views 61
- Downloads 0
Pregnancy, labour, and peurperium of teenage mothers in J. Śniadecki Provincial Hospital of Białystok
Purpose: Pregnancy of teenage girls is of high-risk. It requires special care and concern in the medical, socio-economic, legal, and psycho-pedagogical aspect. The aim of this study was to analyze the incidence of early...
When a doctor becomes a patient, the unique expectations and behaviours in a disease: preliminary report
Descriptions and interpretations of cases in which a doctor becomes a patient are rather marginal in the Polish and Western medical literature. Still, analysis of doctors’ behaviours when they become patients themselves...
Women’s knowledge about cervical carcinoma risk factors and their health behaviours: Study among nurses
Purpose: To determine the level of knowledge regarding cervical carcinoma risk factors and to evaluate selected health behaviours presented by nurses in primary prevention of the reproductive organ malignancies.Materials...
Effects of granulocyte colony-stimulating factor treatment in children and patients with cerebral palsy: a preliminary report
neuroinflammation and apoptosis in brains affected by cerebral palsy could be therapeutic targets. Granulocyte colony-stimulating factor (G-CSF) exerts anti-inflammatory and antiapoptosis effects and stimulates the proli...
Effects of short moderate exercise on hematological parameters and stem cells in healthy humans
Introduction: Exercise at various durations and intensities impact on blood and stem cells. This pilot study examined the effects of 30 minutes of treadmill walking on hematological indices and progenitor stem cells CD34...