CLOT FORMATION AND METHYLENE BLUE REDUCTION IN MILK BASED MEDIUM FOR IDENTIFICATION OF CANDIDA SPP. AND TRICHOSPORON SPP.
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2016, Vol 5, Issue 48
Abstract
Candidiasis is one of the most important opportunistic fungal infections in man caused by over 20 species of Candida. After the genus Candida, Trichosporon is the most commonly reported agent of disseminated infections caused by yeasts. In the routine microbiology laboratory, yeast species are frequently identified on the basis of culture characteristics, germ tube production, carbohydrate fermentation reactions and chlamydospore formation by Dalmau technique. These conventional methods usually lack specificity and require longer incubation period resulting in delayed identification, which can lead to a delay in empirical therapies of such infections. The present study, thus, evaluated the reliability of methylene blue reduction test for the rapid identification of Candida and Trichosporon species. Results indicated that clot formation in milk could be reliably used for speciation of these yeasts.
Authors and Affiliations
Afreen , Sayan , Ashwini , Asim , Abhishek , Dhirendra , Rohit , Shweta
Keywords
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- EP ID EP112596
- DOI 10.14260/jemds/2016/709
- Views 90
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