DETECTION OF HELICOBACTER PYLORI ANTIGEN IN STOOL BY ENZYME-LINKED IMMUNOSORBENT ASSAY AND COMPARISON WITH CONVENTIONAL METHODS
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2016, Vol 5, Issue 51
Abstract
Helicobacter pylori (H. pylori) bacteria are ‘slow’ bacterial pathogens and are associated with gastritis, peptic ulcers, gastric adenocarcinoma and gastric Mucosa-Associated Lymphoid Type (MALT) B-cell lymphomas. Several methods, both invasive and noninvasive, are available for detection of H. pylori infection. Invasive methods involve endoscopy and examination of gastric biopsies, e.g. by culture, rapid urease test or histology and are not appropriate for large-scale population studies. Non-invasive methods include the urea breath test, serology and stool antigen test. The latter approach is non-invasive, does not require highly specialized equipment and unlike serology is more likely to provide evidence of active rather than past infection. Furthermore, it may be more appropriate for use in paediatric patients, where techniques such as serology are insensitive and invasive methods are undesirable. Additionally, it may be used for treatment follow-up purposes. Pathogen-specific stool antigen tests are a valid alternative to the Urea Breath Test for non-invasive detection of H. pylori. METHODOLOGY A total of 120 patients who underwent upper gastrointestinal endoscopy for various gastrointestinal disturbances like dyspepsia were included in the study. Stool samples were obtained from the patient on the day of endoscopy and stored at – 20oC. Three biopsy samples were collected, two from the gastric antrum and one from the corpus. One biopsy sample from the antrum was used for performing Rapid urease test at the Endoscopy room and the other two samples were placed in 10% formalin and sent to the laboratory for histopathological examination. RESULTS Sensitivity, specificity, positive and negative predictive values of ELISA was 100%, 77%, 52% and 100% respectively. CONCLUSION H. pylori stool antigen (HpSA) is suitable to use particularly in developing countries and for selection of patients for endoscopy. Detection of HpSA shows high sensitivity and specificity and might be useful for non-invasive diagnosis of H. pylori infection in children and adult patients.
Authors and Affiliations
Rajesh Selvi , Suraj Shankeregowda, Yuvaraja , Subburaj
Keywords
Related Articles
- EP ID EP107209
- DOI 10.14260/jemds/2016/766
- Views 98
- Downloads 0
A RARE CASE OF DEBILITATING HEADACHE AND ANAPLASTIC OLIGOASTROCYTOMA OF FRONTAL LOBE
PRESENTATION OF CASE A 25-year-old female patient from Mandya, Karnataka came to Ophthalmology OPD at Vasan Eye Care, Bangalore. Complaint of headache since one month. Headache initially was bi-frontal then gradually bec...
ASSOCIATION BETWEEN GALL BLADDER DIAMETER AND CALCULUS IN THE CYSTIC DUCT IN GALL STONE DISEASES AND ITS IMPORTANCE IN THE CURRENT SURGICAL PRACTICE- A CROSSSECTIONAL STUDY IN JNIMS, POROMPAT, MANIPUR
BACKGROUND Gall Stone Disease is still a problem even in this new era. Patients with suspected gall bladder disease visiting Jawaharlal Nehru Institute of Medical Sciences, Porompat, Manipur (JNIMS) were diagnosed by doi...
STUDY ON BACTERIOLOGY OF TONSILLITIS IN A HOSPITAL
[b]OBJECTIVES: [/b]Tonsillitis affects all age groups especially children. Our aim is to find out the main causative agents responsible for causing tonsillitis. [b]METHODS: [/b]The study was performed on 152 patients...
THE ROLE OF PROPHYLACTIC ANTIBIOTICS IN PATIENTS UNDERGOING ELECTIVE CHOLECYSTECTOMY- A RANDOMISED PLACEBO CONTROLLED RECIPIENT BLIND TRIAL
BACKGROUND Laparoscopic cholecystectomy (LC) is the gold standard treatment for symptomatic cholelithiasis. The incidence of infectious complications after LC is significantly lower compared with infections with open cho...
DISTRIBUTION OF BLOOD GROUP IN BLOOD DONOR POPULATION IN WEST BENGAL
BACKGROUND The ABO blood group and Rh type is unique to every single individual. Prevalence of blood groups of all four types is different in various regions of this world. Aim: The present study is intended to detect th...