Development and Validation of a Simple UV Spectrophotometric Method for the Determination of Cefotaxime Sodium in Bulk And Pharmaceutical Formulation
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 1
Abstract
The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefotaxime sodium in bulk and pharmaceutical formulation. The solvent used throughout the experiment was methanol and water. Absorption maximum (λmax) of the drug was found to be 260 nm. The quantitative determination of the drug was carried out at 260 nm and Beer’s law was obeyed in the range of 10-30μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.025x + 0.0028 with correlation coefficient R2 of 0.9995. The recovery values for cefotaxime sodium ranged from 99.95% - 100.21%.The percent relative standard deviation (RSD %) of interday precision range was 0.099 – 0.140% and intraday precision range was 0.098 – 0.132%. The limit of detection and limit of quantification was 0.079μg/mL and 0.154μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.142 – 0.221%. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.
Authors and Affiliations
Most. Umme Bushra , Nahia Akter , Md. Rajib Hassan , Atikul Islam , Md. Radwan Hossain
Keywords
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