DEVELOPMENT AND VALIDATION OF ASSAY METHOD FOR MELOXICAM TABLETS BY RP-HPLC
Journal Title: Int J of Pharm Res & Analy - Year 2016, Vol 6, Issue 1
Abstract
ABSTRACT A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the determination of meloxicam .The separation was carried out using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60: 40. The pH of the mobile phase was adjusted to 7.0 with triethylamine. The column used was X Terra C18 (150 × 4.6 mm, 5 μm) with flow rate of 0.8 mL/min using UV detection at 344nm. The total run time was 6 min and the retention time of meloxicam was 2.4 min. The described method was linear for the assay of meloxicam over a concentration range of 10 μg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The Limit of quantification and Limit of detection were found to be 0.135 μg/mL and 0.05μg/mL respectively. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of meloxicam bulk drug and its pharmaceutical dosage form. Keywords: Meloxicam, HPLC.
Authors and Affiliations
D. China babu, M . Sreenivasulu, M . Naga priyanka
Keywords
Related Articles
- EP ID EP164582
- DOI -
- Views 132
- Downloads 0
FORMULATION AND EVALUATION OF SULPHAMETHOXAZOLE SUSPENSION BY PH CHANGE METHODS
Dispersed phase preparation is an important step in the formulation of suspensions. One of the good criteria for suspension is fine (micron) sized dispersed phase particles. Fine size of the particles in a suspension is...
SUPER CRITICAL FLUID CHROMATOGRAPHY: AN OVERVIEW
Supercritical Fluid Chromatography (SFC) is a form of normal phase chromatography, first used in 1962.SFC typically utilizes carbon dioxide as the mobile phase; therefore the entire chromatographic flow path must be pres...
ATTENUATED TOTAL REFLECTANCE SPECTROSCOPY: AN OVERVIEW
Attenuated Total Reflectance (ATR) is today the most widely used FTIR sampling tool. ATR generally allows qualitative or quantitative analysis of samples with little or no sample preparation which greatly speeds sample a...
FORMULATION AND EVALUATION OF FLOATING DRUG DELIVERY SYSTEM OF ZIDOVUDINE
Zidovudine is considered a good candidate for incorporation in a gastro-retentive dosage form due to its high solubility in the stomach medium compared with its solubility in the small intestine medium. As its solubility...
VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ASSAY OF FUSIDIC ACID, BETAMETHASONE-17 VALERATE AND CHLOROCRESOL CONTENT IN TOPICAL PHARMACEUTICAL FORMULATION
A new stability indicating reversed phase HPLC method was developed and validated for assay of fusidic acid, betamethasone -17 valerate and Chlorocresol in Topical pharmaceutical formulation. The chromatographic separati...