DEVELOPMENT AND VALIDATION OF ASSAY METHOD FOR MELOXICAM TABLETS BY RP-HPLC
Journal Title: Int J of Pharm Res & Analy - Year 2016, Vol 6, Issue 1
Abstract
ABSTRACT A simple, rapid, and accurate reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the determination of meloxicam .The separation was carried out using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60: 40. The pH of the mobile phase was adjusted to 7.0 with triethylamine. The column used was X Terra C18 (150 × 4.6 mm, 5 μm) with flow rate of 0.8 mL/min using UV detection at 344nm. The total run time was 6 min and the retention time of meloxicam was 2.4 min. The described method was linear for the assay of meloxicam over a concentration range of 10 μg/mL respectively. Results of the analysis have been validated statistically and by recovery studies. The Limit of quantification and Limit of detection were found to be 0.135 μg/mL and 0.05μg/mL respectively. The results of the studies showed that the proposed RP-HPLC method is simple, rapid, precise, and accurate, which is useful for the routine determination of meloxicam bulk drug and its pharmaceutical dosage form. Keywords: Meloxicam, HPLC.
Authors and Affiliations
D. China babu, M . Sreenivasulu, M . Naga priyanka
Keywords
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