Dynamics of bacterial community in the gut of Cornu aspersum
Journal Title: Journal of BioScience and Biotechnology - Year 2015, Vol 4, Issue 3
Abstract
The dynamics of the bacterial community in the intestinal tract of Cornu aspersum was investigated during different states of its life cycle. Two approaches were applied – culture and non-culture. The non-culture approach was performed by ARDRA of 16S rDNA using two of the six tested endonucleases. Data were analyzed by hierarchical cluster analysis. The restriction of 16S rDNA samples from the snail of different physiological states with endonucleases HinfI and Csp6I resulted in generation of different profiles depending on the snail states. By the culture approach we found that the total number of cultivable bacteria, representatives of Enterobacteriaceae, lactic acid bacteria, amylolitic and cellulolytic bacteria were the most abundant in active state of the snails. Cellulolytic bacteria were not detected in juveniles of C. aspersum. Escherichia coli, Clostridium perfringens as well as bacteria from the genus Salmonella, Shigella and Pseudomonas were not detected. Bacteria of the genus Aeromonas were found in juveniles of C. aspersum, after that their number decrease and were not found in hibernating snails. On the base of the two applied approaches this study shows that the bacterial flora in the intestinal tract of C. aspersum is affected by the seasonal and environmental variations and undergoes quantitative and qualitative changes during the different states of the life cycle. The snails harbor in their gut intestinal bacteria, which possess biochemical potentiality to degrade the plant components.
Authors and Affiliations
ZDRAVKA KOLEVA, YOANA KIZHEVA, SAVA TISHKOV, IVAYLO DEDOV, EKATERINA KIROVA, PETYA STEFANOVA, PENKA MONCHEVA
Keywords
Related Articles
- EP ID EP148763
- DOI -
- Views 69
- Downloads 0
Breeding potential of the field pea (Pisum sativum L.) cultivars and their progenies
The inheritance and gene effects structure were investigated for ten quantitative traits in field cultivars by a full diallel model of four parents – the cultivars Kerpo, Pleven 10, Mir and EFB33. Overdominance was...
Genetic diversity analysis of chewing sugarcane (Saccharum officinarum L.) varieties by using RAPD markers
In the present study an efficient and easy method was followed for the isolation of DNA from meristem cylinder in five chewing sugarcane varieties, namely Amrita, Bomaby, Babulal (Co.527), Q83 and Misrimala. The quality...
Effect of different levels of Saccharomyces cerevisiae supplementation on in vitro gas production kinetics of some grape yield by-products
The aim of this study was to determine the chemical composition of some grape yield by-products supplemented with Saccharomyces cerevisiae and gas production characteristics using in vitro gas production technique. The t...
Development of bioprocess for high density cultivation yield of the probiotic [i]Bacillus coagulans[/i] and its spores
[i]Bacillus coagulans[/i] is a spore forming lactic acid bacterium. Spore forming bacteria, have been extensively studied and commercialized as probiotics. Probiotics are produced by fermentation technology. There is a l...
Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method
A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – a...