Effect of Lactobacillus species on Streptococcus mutans Biofilm formation
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 5
Abstract
Streptococcus mutans is the primary pathogen responsible for initiating dental caries and decay. The presence of sucrose, stimulates S. mutans to produce insoluble glucans to form oral biofilm also known as dental plaque to initiate caries lesion. The GtfB and LuxS genes of S. mutans are responsible for formation and maturation of biofilm. Lactobacillus species as probiotic can reduces the count of S. mutans. In this study effect of different Lactobacillus species against the formation of S. mutans biofilm was observed. Growing biofilm in the presence of sucrose was detected using 96 well microtiter plate crystal violet assay and biofilm formation by S. mutans in the presence of Lactobacillus was detected. Gene expression of biofilm forming genes (GtfB and LuxS) was quantified through Real-time PCR. All strains of Lactobacillus potently reduced the formation of S. mutans biofilm whereas Lactobacillus acidophilus reduced the genetic expression by 60-80%. Therefore, probiotic Lactobacillus species can be used as an alternative instead of antibiotics to decrease the chance of dental caries by reducing the count of S. mutans and their gene expression to maintain good oral health.
Authors and Affiliations
Ayaz Ahmed , Wu Dachang , Zhou Lei , Liu Jianjun , , Qiu Juanjuan , Xin Yi
Keywords
Related Articles
- EP ID EP137137
- DOI -
- Views 79
- Downloads 0
Toxicological investigations of Aloe ferox Mill. extracts using Brine shrimp (Artemia salina L.) assay
Cytotoxicity of the extracts of Aloe ferox using brine shrimp was evaluated. Effects of the extracts on hatchability and lethality of brine shrimps were reported in terms of minimum inhibitory concentration and LD5...
Hepatoprotective and toxicological studies of Salvia bucharica methanolic extract in rabbits
Most of the species of genus Salvia are famous for having medicinal properties due to their chemical constituents. Salvia bucharica (Lamiacea) is found in Balochistan near Quetta in Hannaurak and Kalat. It is used...
The effect of Centella asiatica, vitamins, glycolic acid and their mixtures preparations in stimulating collagen and fibronectin synthesis in cultured human skin fibroblast
Abstract: Centella asiatica (Linn.) Urban is well known in promoting wound healing and provides significant benefits in skin care and therapeutic products formulation. Glycolic acid and vitamins also play a role in...
Effect of methanolic extract of Asparagus racemosus Willd. on lipopolysaccharide induced-oxidative stress in rats
Lipopolysaccharide (LPS) induced oxidative stress and impairment of normal physiological function generally categorized by increased anxiety and reduced mobility. Therefore, the present study was to find out the&nbs...
In vitro antioxidant effect of Camellia sinensis on human cell cultures
Camellia sinensisis traditionally used in many polyherbal preparations for the treatment of different diseases and infections. Its action has been associated with its antioxidant activities. In this study, antioxid...