Estimation of sulfolaxand antimicrobial preservatives in laxative drops
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 6
Abstract
A simple, fast, precise, economic, selectiveand accurate HPLC method for simultaneous estimation of sorbicacid, sodium picosulphate and methyl parabensodium in laxative drops has been developed and subsequently validated. Chromatographic separation was achieved using gradient elution with mix phosphate buffer pH 7.0 and acetonitrile. The column used was purospherstar C18, 5μm, 25cm × 4.6mm kept at 25°C with 1ml/min flow rate using detection (PDA) at 263nm. The retention times of sorbicacid, sodium picosulphate and methyl paraben sodium were found to be 4.6, 7.4 and 11.4 minutes respectively. The proposed method was found to be linear over a concentration range of 8-12μg/ml for sorbic acid, 60-90μg/ml for sodium picosulphate and 16-24μg/ml formethyl paraben sodium respectively. The recovery was found to be 99.13-101.68% for sorbic acid, 99.81-100.21% for sodium picosulphate and 99.84-100.09% for methyl paraben sodium respectively. The limit of detection (LOD) for sorbicacid, sodium picosulphate and methyl parabensodium were found to be 0.032μg/ml, 0.337μg/ml and 0.131μg/ml respectivelyand limit of quantitation (LOQ) for sorbicacid, sodium picosulphate and methyl parabensodium were found to be 0.097μg/ml, 1.023μg/ml and 0.399μg/ml respectively. The method was validated with respect to specificity, precision, accuracy, linearity and robustness according to guidelines of ICH.
Authors and Affiliations
Muhammad Saqlain Tahir , Rizwan Mahmood , Waseemur Rahman Shamsi
Keywords
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