Ethanolic extract of Nigella sativa protects Fe(II) induced lipid peroxidation in rat’s brain, kidney and liver homogenates
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 1
Abstract
The study describes the effect of ethanolic extract of Nigella sativa against Fe(II) induced lipid peroxidation. Basal and Fe(II) induced thiobarbituric acid reactive species (TBARS) production was significantly inhibited by the ethanolic extract of Nigella sativa at25-200µg/ml. Our data revealed that the extract has high DPPH radical scavenging activity at highest tested concentrations. The extract significantly chelated Fe(II) and scavenged hydroxyl (OH●) radical at 25-200µg/ml concentration. The nutritional analysis was performed and carbohydrate, fats, fiber, protein, moisture and ash content were measured in the studied extract. The phytochemical analysis confirmed the presence of alkaloid, carbohydrate & sugar, glycosides, phenolic compounds, flavonoids, protein and amino acid, phytosterols, tannins, gum and mucilage. The extract also showed significant antimicrobial activities against 10 bacterial strains i.e. Salmonella typhi, Bacillus subtilis, Bacillus cereus, Klebsiella pneumonia, Escheria coli, Xanthomonas, Salmonellaheidelberg, Staphylococcus aureus, Clostridium andEscheria coli (human)and 5 fungal strains i.e.Aspergillusniger, Entomola, Aspergillusflavus, AlternariaalternataandPenicillium. This study confirms the potential antioxidant and antimicrobial activities of ethanolic extract of Nigella sativa which can be considered not only as a diet supplement but can be used against a variety of free radical induced damage diseases
Authors and Affiliations
Waseem Hassan , Hamsa Noreen , ShafqatUllah Khalil , Arshad Hussain , Shakilla Rehman , Shagufta Sajjad , Ata Ur Rahman1 , Joao BT da Rocha
Keywords
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