Extended Spectrum β-Lactamases Producing Klebsiella pneumoniae from the Neonatal Intensive Care Unit at the University Teaching Hospital in Lusaka, Zambia.
Journal Title: Journal of Medical Science and Technology - Year 2015, Vol 4, Issue 2
Abstract
Klebsiella pneumoniae is one of the major causes of blood-stream infections in neonatal intensive care units. Treatment has been a challenge due to the development of multidrug resistant strains, which are mainly attributed to the ability of the organism to produce extended spectrum β-lactamases that confer resistance to second and third generation cephalosporins. This laboratory-based cross-sectional study was aimed at determining the extent of extended spectrum β-lactamase production among invasive K. pneumoniae isolates from blood culture specimens at the University Teaching Hospital in Lusaka. The production of the extended spectrum β-lactamases was detected using the combination disc method, and by detecting genes encoding extended spectrum β-lactamases using Polymerase Chain Reaction. The drug resistance profile was determined using the Kirby-Bauer disc diffusion method against tetracycline, chloramphenicol, amikacin, gentamicin, co-trimoxazole, ciprofloxacin, cefotaxime, ceftazidime, cefpodoxime and imipenem. All the 45 isolates were found to be ESBL producers, and out of these 33/45 SHV gene was detected. Antimicrobial susceptibility testing revealed a high frequency of antimicrobial resistance: cefotaxime
Authors and Affiliations
Geoffrey Kwenda, Enoch Mumbula, Mulemba Samutela, Annie Kalonda, James Mwansa, Darlington Mwenya, Lydia Koryolova, Trevor Kaile, Clemence Marimo, Chileshe Musyan
Keywords
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