First report of Achromobacter xylosoxidans on wheat rhizosphere in Iran and its biocontrol activity
Journal Title: Scientia Agriculturae - Year 2016, Vol 16, Issue 1
Abstract
Achromobacter xylosoxidans belongs to betaproteobacteria, Burkholdriales and Alcaligenaceae. A. xylosoxidans NB1 isolated from wheat rhizosphere in Gorgan, Iran and some of its biocontrol aspects were assay. Biological control has potential for the management of many diseases. In order to Molecular identification, genomic DNA extraction was prepared with CTAB method, and a fragment of 16S rDNA of the bacterium was replicated with PCR that was carried out by using universal primers (27F and 1492R). After electrophoresis, PCR products sequencing was performed by Bioneer Company (South Korea). The nucleotide sequence was assembled with BioEdit, and compared with similar sequences available in NCBI and EzTaxon. Results showed that our strain was most similar to A. xylosoxidans zc1 (100%). Then this bacterium named and registered A. xylosoxidans NB1 with accession number KU504626 in gene bank. NB1 studied for mycelial inhibition of fungi and wheat seeds growth promoting. Biofilm assay showed that this strain has significant biofilm formation. A. xylosoxidans NB1, showed appreciable level of IAA and siderophore production ability. NB1 has good biocontrol activity on Curvularia lunata and Bipolaris sorikiniana and inhibit the mycelia growth and cause inhibition zone. This isolate didn’t have protease, lipase, HCN production and P solubility.
Authors and Affiliations
Negar Bagheri, Masoud ahmadzadeh
Keywords
Related Articles
- EP ID EP101781
- DOI 10.15192/PSCP.SA.2016.16.1.3642
- Views 119
- Downloads 0
Seed Viability And Oxygen Depletion Rate Of Hermetically Stored Maize Infested By Major Insect Pests
Grain moisture content assessment, oxygen depletion rate and seed viability tests were conducted to ascertain the particular type of bag that prolonged germination rate despite infestation by major destructive inse...
Effects of Iron and Zinc on Sweet Lime (Citrus limmetta) Ffruit Quantity and Quality in Calcareous Soil
This study was conducted on 5-years-old grafted sweet lime trees as factorial arrangement in randomized complete block design with 4 replicates in Jahrom region. The first factor was iron sulfate (0, 5 and 10 mgL-1...
Studies on the Impact of cadmium on Growth, Yield Attributes, Yield and Biochemistry of Mung Bean (Vigna radiata L Wilczek) Under Natural Field Condition, Burdwan, West Bengal.
Vigna radiata L. Wilczek is a staple food crop in terms of its high protein value. In our study we investigated in realistic field conditions the effect of six different concentration of cadmium (0,0.05,0.5,1,,10 a...
Effects of Transesterification on the Colour of Biodiesel
Vegetable oils and animal fats are triglycerides, which is a glyceride on to which are attached three free fatty acids. During transesterification, the free fatty acids are removed and replaced with radicals from t...
Comparative analysis of phenotypic characterization of Kenya and Pacific Islands tarogen germplasm collections Colocasia esculenta L. (Schott)
Fifty accessions of taro germplasm collections Colocasia esculenta L. (Schott) were collected from Kenya and Pacific Islands tarogen germplasm collections. Twenty five accessions of Kenyan taro germplasm were colle...