Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time
Journal Title: RSBO - Year 2012, Vol 9, Issue 1
Abstract
Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed.
Authors and Affiliations
Ricardo Silva, Rodrigo Quiezi, Claudia Bertolacini, Suzana Carvalho, Kellen Gasque, Carina Silva, Lucilene Bicudo
Keywords
Related Articles
- EP ID EP155784
- DOI -
- Views 186
- Downloads 0
Tratamento odontológico em pacientes com doença cardiovascular
Introduction: The correct evaluation of patients with cardiovascular disease is based on a careful anamnesis and on the previous knowledge of the main coronary diseases and its sequels. Objective: This article revised th...
Atypical appearance of epidermoid cyst in tongue’s ventral surface
Introduction: Epidermoids cysts are benign and rare lesions in oral cavity. It presents as a nodular lesion of sessile base, soft to palpation. In the oral cavity, it most happens on the floor of the mouth’s midline. Wit...
Effectiveness of the temporary sealing of three restorative materials to the 50% silver nitrate solution
Introduction and objective: The aim of this study was to analyze three temporary restorative materials – Coltosol®, Cavit® W and Cimpat® –, concerning their sealing capacity to the 50% silver nitrate solution. Mate...
Comparison of canal transportationand centering ability of hand Protaperfiles and rotary Protaper files by using micro computed tomography
Introduction and objective: The aim of the present study was to compare root canal preparation with rotary ProTaper files and hand ProTaper files to find a better instrumentation technique for maintaining root cana...
The effects of sexual hormone factors in periodontal tissues
The authors present a clinical case of gingivitis associated to dental plaque, probably influenced by sexual hormones (puberty) factors. Clinical aspects,as well as the results after adequate, treatment are approached in...