Hypoglycemic, Hepatoprotective and Nephroprotective Effects of Methanolic Leaf Extract of Heinsia crinita (Rubiaceae) in Alloxan-induced Diabetic Albino Wistar Rats
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2014, Vol 4, Issue 1
Abstract
Type 1 diabetes is associated with damage to the liver, kidney and pancreas of patients. The damage varies in proportion and susceptibility among diabetic patients of type 1 class. This study assessed the hypoglycemic, hepatoprotective and nephroprotective activities of whole methanolic leaf extract of Heinsia crinita (HC) in alloxan-induced diabetic albino Wistar rats. Twenty four (24) rats weighing 140-180g were divided into 4 groups of 6 rats each. Group 1 served as normal control (NC), while Group 2 served as diabetic control (DC) and both received placebo treatment consisting of 0.2ml of 50% DMSO respectively. Group 3 was treated with standard insulin (5iu/kg b.w) and Group 4 was treated with 400mg/kg b.w of the methanol extract of Heinsia crinita (HC) twice daily via oral gastric intubation. After 14 days, the animals were sacrificed and serum was analyzed for glucose, oxidative stress biomarkers and electrolyte profile. The results showed that HC reduced serum glucose level by 15% within 14 days, while the DC showed no reduction. The results further showed significant (p<0.05) decrease in ALT, AST and ALP activity in groups 4 and 3, when compared with group 2 (DC) which gave high values of these enzymes. Group 4 showed high values of K+(10.93+0.10 Mmol/l) and Cl- (353.23+0.34 Mmol/l) but lower Na+(71.06+12.94 Mmol/l) and urea (66.04+6.06Mmol/l) values when compared to DC with low values of K+(3.83+0.82 Mmol/l) and Cl- (127.09+14.43 Mmol/l) but high values of Na+(203.00+14.47 Mmol/l) and urea (86.00+14.13 Mmol/l) respectively. Electrolyte modulation by the plant extract was shown in this study to be better than insulin. These findings showed that Heinsia crinita may possess antidiabetic properties and beneficial in the management of Type 1 diabetes and its attendant liver and kidney complications.
Authors and Affiliations
1, Ebong, P. E. , 2, Igile, G. O. , 3, Mgbeje, B. I. A. , 4, Iwara, I. A. , 5, Odongo, A. E; 6, Onofiok, U. L. , 7, Oso, E. A. ,
Keywords
Related Articles
- EP ID EP152085
- DOI -
- Views 59
- Downloads 0
[b] Comparative study of spirometric parameters between active tobacco smokers and tobacco non-smokers[/b]
Hepatoprotective Activity of Flavonoids Purified and Ethanolic Extract from Iraqi Propolis Against Carbon tetrachloride-Induced Liver Damage In Male Mice
The present study aims to investigatethe antioxidant activity of flavonoids purified and methanolic extract from propolis on male mice compared to that caused by carbon tetrachloride as a hepatotoxic model by measu...
Synthesis of 2-[{4-(t-amino-1-yl) but-2-yn-1-yl }oxy]-1,3-benzothiazole derivatives as H3-antagonists
Aminoacetylenicoxybenzothiazole derivatives were synthesized from the reaction of 2- hydroxybenzothiazole with 3-bromoprop-1-yne to generate 2-(prop-2-ynl-1-yloxy)-1,3-benzothiazole (RM-1). A mixture of 2-(prop-2-...
Antibiogram and Plasmid Profile of Escherichia Coli Isolates in Well Water In Akure, South Western Nigeria
Well water samples sourced from Akure, Ondo State, Nigeria were analyzed using standard and analytical methods to determine the level of divergence of Escherichia coli, in the well water, its antibiogram and the...
Phytochemical Evaluation And Pharmacological Screening Of Antiparkinson’s And Laxative Activities Of Hylocereus Undatus (White Pitaya) In Rodents
Objective: To evaluate the antiparkinson’s and constipation activity of Hylocereus undatus Haw. fruit extract in haloperidol induced parkinson’s disease and loperamide induced constipation in mice. Method: Parkinson’s di...