„IN VITRO” MULTIPLICATION OF CHRYSANTHEMUM MORIFOLIUM RAMAT.
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2005, Vol 51, Issue 0
Abstract
The tissue-cultured shoot primordia have distinct characteristics of high stability of chromosome number, high ability of regeneration and rapid proliferation. Rapid propagation of Chrysanthemum morifolium Ramat. “Prince de Monaco” and “Romica” was achieved through tissue culture technique. The procedure involved aseptic culture of shoot tips followed by rapid shoot multiplication, rooting and finally establishment of plantlets in soil. The agar solidified MS medium containing 2 mg/l BAP and 0,002 mg/l NAA was optimum for rapid mass production of plantlets. These can be rooted on MS medium in the absence of growth regulators. The indirect micropropagation of Chrysanthemum morifolium Ramat. was achieved through callus cultures obtained from single-node stem segments excised from other two cultivars of Chrysanthemum morifolium Ramat.: “Escorte” and “La Cagouille”, belonging to the collection of Botanical Garden from Iasi.
Authors and Affiliations
SMARANDA VÂNTU
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