INFLUENCE OF GENOTYPE AND CULTIVATION CONDITIONS ON VITROPLANTLETS EVOLUTION OF SOLANUM TUBEROSUM L. LOCAL VARIETIES
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2011, Vol 57, Issue 2
Abstract
The local potato varieties from Gene Bank Suceava are maintained in a field collection and also by slow growth of in vitro culture. Slow growth method allows limited development of plantlets and the extension of time between two subcultures. This paper presents a part of the results about the influence of genotype, inhibitors from the media and conditions from the conservation room (temperature and light reduction), on potato plantlets preserved in vitro for different periods. Three grows inhibitors (daminozide, mannitol and sorbitol) and five local potato varieties of were included in the experiments. Several morphological features: number, length and branching of shoots, rooting, number of viable nodes, number and size microtubers and the viability rate of the shoots, were evaluated. Although there were differences in the growth type of potato varieties the main effect was the reduction of plantlets height, by shortening of internodes and leaves size. The leaf blade became very short (1,5 – 2 mm), many of them growing on the surface of some branches having a hypertrophic and translucent aspect. Could be noted a tendency to generate, also, well-defined microtubers bearing microbranches. The results on the response of plantlets developed on storage media recommended the medium C24, with 40 g / l sorbitol, as the best in terms of in vitro conservation of local potato varieties.
Authors and Affiliations
IUSTINA CIOBANU, DANA CONSTANTINOVICI, L. CREŢU
Keywords
Related Articles
- EP ID EP114051
- DOI -
- Views 93
- Downloads 0
CHOROLOGICAL AND ECOLOGICAL ASPECTS OF NEPETA NUDA L. SSP. NUDA (SYN. N. PANNONICA L.) FROM LAMIACEAENEPETOIDEAE IN ROMANIA
The paper presents chorological and ecological aspects concerning Nepeta nuda L. ssp. nuda species (syn. Nepeta pannonica L.) from Lamiaceae, Nepetoideae. Nepeta nuda is a common species widespread throughout Roman...
CONTRIBUTIONS TO THE STUDY OF THE VASCULAR FLORA AND THE VEGETATION FROM SOME FOREST-STEPPE MEADOWS OF MOLDAVIA (ROMANIA)
Le travail présente la flore vasculaire et la végétation, la chorologie et l’écologie de quelques éspèces des prairies de sylvosteppe de Moldavie (Roumanie). Quelques éspèces sont dans la liste rouge des plantes va...
ARAUCARIA EXCELSA L. VITROCULTURES INITIATION
Araucaria excelsa L. is a well-known conifer, mostly used as an indoor ornamental plant. For the initiation of Araucaria excelsa L. vitrocultures, we have studied the reactions of explants, in the presence of diffe...
EVALUATION OF CYTOTOXIC AND GENOTOXIC POTENTIAL OF THE FUNGICIDE RIDOMIL IN ALLIUM CEPA L.
The cytogenetic effects exerted by the systemic fungicide mefenoxam and copper hydroxide (the active ingredients of Ridomil Gold Plus 42,5 WP fungicide) were studied in root tips of Allium cepa L. A progressive concentra...
QUALITATIVE STUDIES ON THE PRODUCTION OF STEVIOSIDE IN IN VITRO CALLUS CULTURE OF STEVIA REBAUDIANA BERTONI
Stevioside is a glycoside of the diterpene derivatives of steviol. Steviol glycosides are natural constituents of the plant Stevia rebaudiana Bertoni belonging to the Compositae family. The leaves of S. rebaudiana...