Influence of nitrogen compounds on growth and the nitric oxide (NO) content in roots of etiolated pea seedlings
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2012, Vol 8, Issue 4
Abstract
The data on the influence of different concentration of sodium nitroprusside (SNP), potassium nitrate (KNO3), sodium nitrite (NaNO2) and L-arginine on 3 days-old etiolated pea seedlings growth and nitric oxide (NO) content in roots of these seedlings are obtained. It is found that 24 h exposition of seedlings to SNP showed negative influence on growth at a 4 mM dose; SNP at 0.05 mM doze stimulates their growth and 0.1 mM doze did not influence on seedlings growth. Using KNO3 negative influence on growth at the same exposition was showed only at superhigh concentration – 20 mM. Using NaNO2 negative influence on growth was shown at a doze of 2 mM but concentration of 0.1 and 0.5 mM did not influence on it. The exposition of seedlings to L-arginine caused growth inhibition already at concentration of 0.5 mM and reached maximum at 4 mM doze. Determination of NO level in roots using fluorescent probe DAF-2DA in variants with the greatest growth inhibition has showed, that the maximal inhibition of growth in roots and highest level of NO in roots in variants with SNP (4 mM) and NaNO2 (2 mM) was observed. In variants with KNO3 (20 mM) and L-arginine (4 mM) maximal growth inhibition did not coincide with NO accumulation and was observed after 30 min after the beginning of plants exposition and further it was reduced in 24 h. Results are discussed in connection with possible influence NO and participation of the investigated connections in generation nitric oxide in roots of pea seedlings.
Authors and Affiliations
A. Glyan’ko, N. Mitanova
Keywords
Related Articles
- EP ID EP125093
- DOI -
- Views 168
- Downloads 0
Assessment of Actinobacteria Role in Activity of Deep-water Endemic Amphipod Species Belonging to the Genus Ommatogammarus
The aim of present study was to reveal the associations between the actinobacteria strains and Lake Baikal deep-water endemic amphipods. During the study forty-two actinobacteria strains were isolated from the amphipods...
Phytoremediation of Lead and Cadmium Contaminated Soils using Sunflower Plant
Phytremediation has emerged as a practical approach to clean up metal-polluted soils. In this study the role of sunflower (Helianthus annuus L.) plants as a potential phytoremediator to soils contaminated with cadmium (C...
The Effects of Nitrate and Phosphate Deficiencies on Certain Biochemical Metabolites in Tomato (Lycopersicon esculentum Mill. c.v. Urbana V.F.) Plant
Nitrogen (N) and phosphorus (P) are two important macronutrients with diverse functions in plants. Therefore, the effects of their deficiencies on different physiological and biochemical characteristics especially in cro...
РОЛЬ СЛАБОСВЯЗАННЫХ С КЛЕТОЧНОЙ СТЕНКОЙ ПЕРОКСИДАЗ В УСТОЙЧИВОСТИ КАРТОФЕЛЯ ПРИ ИНФИЦИРОВАНИИ КОЛЬЦЕВОЙ ГНИЛЬЮ
Выявлена роль слабосвязанной с клеточной стенкой пероксидазы в определение устойчивости картофеля к действию патогена. Показано, что добавление к суспензионным клеткам картофеля устойчивого сорта Луговской кроличьих ант...
The Responses of Ascorbate - Glutathione Cycle Enzymes in Seedlings of Pancratium maritimum L. under Drought Treatments
In this study, physiological and biochemical responses of (Pancratium maritimum L.), desert plant which is very widespread on coastal sand dunes to drought were determined. Therefore 28 days (d) old plants were drought s...