Influence of short- and long-term exposition to Roundup on the spontaneous and hormonally stimulated LH secretion and gills condition in Prussian carp (Carassius gibelio)
Journal Title: Advances in Environmental Sciences - International Journal of the Bioflux Society - Year 2012, Vol 4, Issue 3
Abstract
Roundup is the most extensively used herbicide in world agriculture. In the present study we examined the influence of Roundup at the concentration of 15 mg L-1on luteinizing hormone (LH) secretion and reproduction of Prussian carp females. Experiment was conducted at the University of Agriculture, Department of Ichthyobiology and Fisheries in Kraków, Poland from May to October 2010. Control fish were kept in Roundup–free water. Short-term exposition lasted 10 days, and long-term exposition 6 months. At the end of both experiments samples of blood were collected and all fish were injected with salmon GnRH analogue (D-Arg6 sGnRH-A, 10 µg kg-1of b.w.) and pimozide (dopamine antagonist, 5 mg kg-1of b.w). After injection blood samples were collected at 6, 12 and 24 hours. LH levels were measured by the ELISA. For histopathological analyses gill samples were stained with H&E method. Analysis of LH concentrations did not show any significant differences between LH levels neither in experimental nor control group at both times of exposition. However the pathological changes in gills in fish from experimental groups in short and long-term exposition were found.
Authors and Affiliations
Grzegorz Gosiewski, Magdalena Socha, Elżbieta Nowak, Jacek Kuchinka, Mirosława Mikołajczyk
Keywords
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