IR INTERPRETATION, DERIVATIVE UV SPECTRA AND VALIDATED UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF HALOPERIDOL IN TABLET DOSAGE FORM
Journal Title: Int J of Preclin & Pharm Res - Year 2013, Vol 3, Issue 1
Abstract
ABSTRACT A simple, accurate and rapid UV method was validated with derivative spectra for the estimation of haloperidol in tablets. The drug is soluble in 0.1 N methanolic sulphuric acid and all the solutions were prepared using the same. Infra-red spectral interpretation by attenuated total reflectance method was done to confirm the structure of haloperidol. The peaks obtained at 1594 cm -1 , 1869 cm -1 , 600-800 cm -1 , 1312 cm -1 , 1681 cm -1 confirm the presence of functional groups like tertiary amine, fluorine, chlorine, hydroxyl and ketone group present. The drug has maximum absorption at 243 nm with a linearity range of 10-50 µg/ml. The %RSD values of accuracy and precision was found to be less than 1.The limit of detection and limit of quantitation were calculated statistically based on the slope of the curve and was found to be 3.8 and 10.6µg/ml respectively. The solution of haloperidol was found to be stable for 8 h after which there was decrease in absorbance. The first, second and third derivative spectra revealed that the peak obtained with the formulation has no interference from other ingredients present. The present study was validated according to ICH guidelines. The proposed analytical method was found to be accurate, precise, and reproducible. Keywords: Haloperidol, UV spectroscopy, IR interpretation, Derivative spectra.
Authors and Affiliations
R. K. Sangeetha*, T. Sudhakaran , T. K. Ravi , M. Mahendra Raja
Keywords
Related Articles
- EP ID EP117929
- DOI -
- Views 110
- Downloads 0
AN OVERVIEW ON FOURIER TRANSFORM MASS SPECTROMETRY
ABSTRACT This article provides an introduction to Fourier transform-based mass spectrometry. The key performance characteristics of Fourier transform-based mass spectrometry, mass accuracy and resolution, are prese...
A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ELETRIPTAN BY USING RP-HPLC METHOD
ABSTRACT A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for estimation of eletriptan in tablet dosage form. Chromatographic separation was...
AN OVERVIEW ON HIGH PERFORMANCE LIQUIDCHROMATOGRAPHY- MASS SPECTROSCOPY
ABSTRACT Ultra high performance liquid chromatography\ms is an extremely versatile instrumental technique. As the name suggested the instrumentation comprises a high performance liquid chromatography attached, via...
IR INTERPRETATION, DERIVATIVE UV SPECTRA AND VALIDATED UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF HALOPERIDOL IN TABLET DOSAGE FORM
ABSTRACT A simple, accurate and rapid UV method was validated with derivative spectra for the estimation of haloperidol in tablets. The drug is soluble in 0.1 N methanolic sulphuric acid and all the solutions wer...
IN VITRO CONTROL OF SELECTED PATHOGENIC ORGANISMS BY METHANOLIC-AQUA EXTRACT OF ACANTHOSPERMUM AUSTRALE LEAVES
ABSTRACT The current study was done to analyse the antibacterial activity of the methanolic- aqua extract of Acanthospermum australe. From the study, the plant was found to inhibit only Bacillus cereus (20.00± 0...