IR INTERPRETATION, DERIVATIVE UV SPECTRA AND VALIDATED UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF HALOPERIDOL IN TABLET DOSAGE FORM

Journal Title: Int J of Preclin & Pharm Res - Year 2013, Vol 3, Issue 1

Abstract

 ABSTRACT A simple, accurate and rapid UV method was validated with derivative spectra for the estimation of haloperidol in tablets. The drug is soluble in 0.1 N methanolic sulphuric acid and all the solutions were prepared using the same. Infra-red spectral interpretation by attenuated total reflectance method was done to confirm the structure of haloperidol. The peaks obtained at 1594 cm -1 , 1869 cm -1 , 600-800 cm -1 , 1312 cm -1 , 1681 cm -1 confirm the presence of functional groups like tertiary amine, fluorine, chlorine, hydroxyl and ketone group present. The drug has maximum absorption at 243 nm with a linearity range of 10-50 µg/ml. The %RSD values of accuracy and precision was found to be less than 1.The limit of detection and limit of quantitation were calculated statistically based on the slope of the curve and was found to be 3.8 and 10.6µg/ml respectively. The solution of haloperidol was found to be stable for 8 h after which there was decrease in absorbance. The first, second and third derivative spectra revealed that the peak obtained with the formulation has no interference from other ingredients present. The present study was validated according to ICH guidelines. The proposed analytical method was found to be accurate, precise, and reproducible. Keywords: Haloperidol, UV spectroscopy, IR interpretation, Derivative spectra.

Authors and Affiliations

R. K. Sangeetha*, T. Sudhakaran , T. K. Ravi , M. Mahendra Raja

Keywords

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  • EP ID EP117929
  • DOI -
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How To Cite

R. K. Sangeetha*, T. Sudhakaran, T. K. Ravi, M. Mahendra Raja (2013).  IR INTERPRETATION, DERIVATIVE UV SPECTRA AND VALIDATED UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF HALOPERIDOL IN TABLET DOSAGE FORM. Int J of Preclin & Pharm Res, 3(1), 18-21. https://europub.co.uk/articles/-A-117929