Mitochondria as a Possible Place for Initial Stages of Steroid Biosynthesis in Plants[sub][/sub]
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2014, Vol 10, Issue 4
Abstract
With the aim of thorough comparison of steroidogenic systems of plants and animals, transgenic plants of Solanaceae family expressing [i]CYP11A1 [/i]cDNA encoding cytochrome P450SCC of mammalian mitochondria were further analysed. Positive effect of [i]CYP11A1 [/i]on resistance of the transgenic tobacco plants to the infection by fungal phytopathogene Botrytis cinerea was for the first time detected. Subtle changes in mitochondria of the transgenic Nicotiana tabacum plants expressing mammalian [i]CYP11A1[/i] cDNA were demonstrated by transmissive electron microscopy. The main components of the electron transfer chain of plant mitochondria were for the first time cloned and characterized. It was established that plants from the Solanacea family (tomato, tobacco and potato) contain two different genes with similar exon-intron structures (all contain 8 exons) encoding mitochondrial type ferredoxins (MFDX), and one gene for mitochondrial ferredoxin reductase (MFDXR). The results obtained point out on profound relatedness of electron transfer chains of P450-dependent monooxygenases in mammalian and plant mitochondria and support our previous findings about functional compatability of steroidogenic systems of Plantae and Animalia.
Authors and Affiliations
Elena Shematorova, Ivan Slovokhotov, Marat Khaliluev, Irina Berdichevets, Ekaterina Baranova, Olga Babak, Dmitry Shpakovski, Svetlana Spivak, George Shpakovski
Keywords
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