Myostatin gene (MSTN) polymorphism with a negative effect on meat productivity in Dzhalginsky Merino sheep breed
Journal Title: Journal of BioScience and Biotechnology - Year 2015, Vol 4, Issue 2
Abstract
One of the most important negative regulator of muscle grow in mammalians is myostatin. Some mutations in myostatin gene (MSTN) can decrease the effect of protein and play role in meat quality of sheep. Therefore, in genome selection, knowledge of MSTN gene structure is very important. We investigated the polymorphism of the MSTN gene and its influence on body parameters in Russian sheep breed Dzhalginsky Merino. To detect alleles, we use NimbleGen sequencing technolog). In this breed, we found 20 single nucleotide polymorphism (SNP). That is SNP in promoter: с.-1866, с.-1404, с.-1401, с.-1213, с.-1128, с.-958, с.-783; 5'UTR: с.-40; exon I: с.101; intron 1-2: c.373+18, c.373+241, c.373+243, c.373+259, c.373+563; intron 2-3: с.747+164, с.747+309, с.748-810, с.748-229G>A, с.748-475; 3'UTR: с.*1232. Three of detected SNP (c.-1128, c.-958, c.-40) have a negative effect on the body parameters – decrease weight, height and other. Other three SNP (c.101, c.373+18, с.*1232) have not significant influence on this parameters. Our investigation is a base of next research of affection of different MSTN gene alleles on meat quality and can be used to prepare a PCR test-system for genomic selection.
Authors and Affiliations
VLADIMIR TRUKHACHEV, VALERY BELYAEV, ANDREY KVOCHKO, ALEXANDER KULICHENKO, DMITRY KOVALEV, SERGEY PISARENKO, ANNA VOLYNKINA, MARINA SELIONOVA, MAGOMET AYBAZOV, SVETLANA SHUMAENKO, ARSLAN OMAROV, TATYANA MAMONTOVA, NATALIA GOLOVANOVA, OLESYA YATSYK
Keywords
Related Articles
- EP ID EP143043
- DOI -
- Views 96
- Downloads 0
Effects of antibiotic growth promoter, probiotic and basil essential oil supplementation on the intestinal microflora of broiler chickens
Effects of the probiotic ‘Protexin', basil essential oil and the antibiotic growth promoter ‘Avilamycin' were studied on the ileum microbial flora of broilers when these substances are used as broiler feed additive...
Sensitivity of watermelon variety Bojura to m[sup][/sup]utant agents [sup]60[/sup]Co and EMS[sup][/sup]
A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with [sup]60[/sup]Co gamma rays (80...
Milk protein tailoring to improve functional and biological properties
Proteins are involved in every aspects of life: structure, motion, catalysis, recognition and regulation. Today's highly sophisticated science of the modifications of proteins has ancient roots. The tailoring of proteins...
Growth and development of saffron (Crocus sativus L.) in response to temperature pre-treatment and environmental conditions
Climatic indices, such as temperature, have an important role in saffron yield. This study was undertaken to investigate the effect of temperature-controlled conditions on the growth and development of saffron. For this...
Sibling vole species (Microtus) in the fragmented landscape of south-eastern part of Thrace, Balkan Peninsula: species presence, habitat selection and craniometry
The presence of sibling vole species (Microtus) in the most common forests and open landscapes in south-eastern part of Thrace (Balkan Peninsula) of contemporary territories of Bulgaria and Turkey was studied. Only...