Over expression of a synthetic gene encoding interferon lambda using relative synonymous Codon usage bias in Escherichia coli
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 6
Abstract
Interferon Lambda (IFN-λ) is a type III interferon which belongs to a novel family of cytokines and possesses antiviral and antitumor properties. It is unique in its own class of cytokines; because of the specificity towards its heterodimer receptors and its structural similarities with cytokines of other classes. This renders IFN-λ a better choice for the treatment against many diseases including viral hepatitis and human coronavirus (HCoV-EMC). The present study describes a computational approach known as relative synonymous codon usage (RSCU); used to enhance the expression of IFN-λ protein in a eukaryotic expression system. Manually designed and commercially synthesized IFN-λ gene was cloned into pET-22b expression plasmid under the control of inducible T7-lac promoter. Maximum levels of IFN-λ expression was observed with 0.4 mM IPTG in transformed E. coli incubated for 4 hours in LB medium. Higher concentrations of IPTG had no or negative effect on the expression of IFN-λ. This synthetically over expressed IFN-λ can be tested as a targeted treatment option for viral hepatitis after purification.
Authors and Affiliations
Hashaam Akhtar , Samar Akhtar , Syed Umer Jan , Azka Khan , Najam us Sahar Sadaf Zaidi , Ishtiaq Qadri
Keywords
Related Articles
- EP ID EP109803
- DOI -
- Views 92
- Downloads 0
Evaluation and comparison of different brands of domperidone tablets available in Karachi, Pakistan
Domperidone is an anti-dopaminergic drug used for the treatment of nausea, vomiting and dyspepsia. It has also been used in Parkinson’s disease. In this study, five different brands of Domeperidone tablets were sel...
Synthesis, characterization and biological evaluation of some 5-methylpyrazine carbohydrazide based hydrazones
Pyrazine carbohydrazide based hydrazones were synthesized starting from 5-methylpyrazine-2-carboxylic acid. The acid was first converted to its methyl ester, which on further treatment with hydrazine hydrate transf...
RP-HPLC analytical method development and optimization for quantification of donepezil hydrochloride in orally disintegrating tablet
An easy, fast and validated RV-HPLC method was invented to quantify donepezil hydrochloride in drug solution and orally disintegrating tablet. The separation was carried out using reversed phase C-18 column (Agilen...
Effect of renin inhibition on adipokines in diabetic rats
Insulin resistance predicts development of type 2 diabetes mellitus (DM). Adipocytes release tumor Necrosis factor-alpha (TNF-α), and adiponectin. They modulate whole-body insulin sensitivity . The disturbance in t...
Real time and accelerated stability studies of Tetanus toxoid manufactured in public sector facilities of Pakistan
Tetanus is an acute illness represented by comprehensive increased inflexibility and spastic spasms of skeletal muscles. The poor quality tetanus toxoid vaccine can raise the prevalence of neonatal tetanus. WHO has...