Phylogenetic tree construction based on amino acid composition and nucleotide content of complete vertebrate mitochondrial genomes
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2013, Vol 3, Issue 6
Abstract
To evaluate the appropriateness of phylogenetic trees in biological evolution, we expanded a pre-existing baseline data set of randomly selected organisms by incorporating a collection of intentionally chosen organisms. Using two different clustering algorithms—Ward’s method and neighbor-joining—we constructed phylogenetic trees based on nucleotide sequences as well as amino acid composition and nucleotide content of complete mitochondrial genomes and 16S rRNA and NADH dehydrogenase subunit 5 genes. We compared classifications derived from cluster analyses with data based on mathematical calculations of complete mitochondrial genomes. Analyses of predicted amino acid composition from complete mitochondrial genomes and of 16S rRNA sequences clearly differentiated terrestrial and aquatic vertebrates. Although no truly representative phylogenetic tree exists, phylogenetic trees provide scientifically appropriate information about biological evolution.
Authors and Affiliations
Kenji Sorimachi Teiji Okayasu
Keywords
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