Phytochemical, antimicrobial, insecticidal and brine shrimp lethality bioassay of the crude methanolic extract of Ajuga parviflora Benth
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 4
Abstract
Methanolic extract of medicinal herb Ajuga parviflora Benth. was evaluated for phytochemical screening (the plant extract showed the presence of aromatic compounds, carbohydrates, glycosides, tannins, alkaloids, polyphenols, quinines and dions, aminophenols, steroids/sterols, flavonoids and terpenoids), antimicrobial activities against various strains of bacteria and fungi by using disc diffusion method and insecticidal activities against red flour beetle (Tribolium castaneum), wheat weevil (Sitophilis granaries) and their larvae. The crude extract showed anti-bacterial activity against all strains with a maximum zone of inhibition of 12mm diameter against Citrobacter and Pseudomonas aurogenosa. Standard drugs Ampicillin, Gentamicin and Amoxicillin were used in parallel. The crude extract did not show antifungal activity against the tested strains of fungi even at high doses. The crude methanolic extract was also used for insecticidal activity against the two types of insects and their larva. The extract showed no significant mortality in the tested strains. For brine shrimp lethality bioassay different concentrations 10, 100 and 1000ug/ml of the medicinal herb A. parviflora methanolic extract were used. After 24 hrs the percent mortality and LD50 value was calculated through probit analysis. The LD50 value of extract was 321.42µg/ml while that of standard drug cyclophosphamide was 16.09ug/ml.
Authors and Affiliations
Najmur Rahman, Mansoor Ahmad, Muhammad Riaz, Mehjabeen , Noor Jahan, Rizwan Ahmad
Keywords
Related Articles
- EP ID EP156731
- DOI -
- Views 111
- Downloads 0
Attenuation of methylphenidate-induced tolerance on cognition by buspirone co-administration
Methylphenidate as a psycho stimulant drug has been prescribed in neuropsychiatric disorders to increase cognition and attention therefore is a medication of choice for attention-deficit/hyperactivity disorder howe...
Anti-glycationand Anti-oxidation properties of Capsicum frutescens and Curcuma longa fruits: Possible role in prevention of diabetic complication
The accumulation of advanced glycationend products (AGE’s) in the body, due to the non-enzymatic glycation of proteins is associated with several pathological conditions like aging and diabetes mellitus. Hence a pl...
Antimicrobial activities of three species of family mimosaceae
The antimicrobial activities of crude methanolic extract of leaves of Acacia nilotica L., Albizia lebbeck L. and Mimosa himalayana Gamble belonging to family mimosaceae were investigated in this research work. Antibacter...
Haloperidol-induced extra pyramidal symptoms attenuated by imipramine in rats
Effects of administration of imipramine (IMI) are determined on haloperidol-induced extrapyramidal symptoms (EPS). Haloperidol is administered orally at a dose of 0.2 mg/rat/day in rats for a period of 5 weeks, by...
Anti-ulcer activity of the aqueous extract of Melastoma malabathricum L. leaf in rats
Melastoma malabathricum L. Smith (Melastomaceae) has been used in the Malay traditional culture to treat ulcer-based ailments. The objective of the present study was to investigate the potential anti-ulcer effect o...