Prophylactic role of B vitamins against bulk and zinc oxide nano-particles toxicity induced oxidative DNA damage and apoptosis in rat livers
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 1
Abstract
The aim of this work is to explore the protective of B vitamins (B3, B6 and B12) against the hepatotoxic potency of either bulk zinc oxide (ZnO-bulk) or its nanoparticles (ZnO-NPs)-induced liver damage in rats. ZnO- bulk or its NPs were administered orally (500 mg/kg b.w.) for 10 successive days. The results revealed that oral co-administration of combination of B vitamins (250 mg B3, 60 mg B6 and 0.6 mg B12 /Kg body weight) daily for 3 weeks to rats intoxicated by either ZnO- bulk or its NPs markedly ameliorated increases in serum of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehdrogenase (LDH). The B vitamins also down-regulated increases in serum glucose level as well as increases in immuno-inflammatory biomarkers, including tumor necrosis factor-α (TNF- α) and C-reactive protein compared with intoxicated, untreated rats. Beside, the used agent successfully modulated the alterations in serum vascular endothelial growth factor (VEGF), attenuated liver oxidative DNA damage compared with ZnO intoxicated groups. We showed that the used B complex mitigated increased malondialdehyde (MDA), decrease in glutathione peroxidase (GPx) and increase in the apoptosis marker caspase 3 of liver tissue in response to either ZnO- bulk or its NP toxicity. In conclusion, early treatment with vitamin B complex may protect liver tissue from deleterious damage induced by the toxic effects of ZnO- bulk or its NPs.
Authors and Affiliations
Jehad Mustafa Yousef , Azza Mostafa Mohamed
Comparative clinical efficacy and safety of coded herbal medicine dermovix in the management of patients with atopic dermatitis versus allopathic medicine
Atopic dermatitis (AD) is defined as a chronic, highly pruritic inflammatory condition of skin. It is estimated that this disease may lead significant morbidity and also adversely affects the quality of life. Atopi...
Oral administration of haloperidol at clinically recommended doses elicits smaller parkinsonian effects but more tardive dyskinesia in rats
The present study was designed to monitor extrapyramidal symptoms (EPS) elicited by the oral administration of haloperidol at clinically recommended doses and to compare it with EPS produced when the drug is injected int...
Antiproliferative xanthone derivatives from Calophyllum inophyllum and Calophyllum soulattri
Structure-activity relationships of eleven xanthones were comparatively predicted for four cancer cell lines after the compounds were subjected to antiproliferative assay against B-lymphocyte cells (Raji), colon ca...
Comparative evaluation of in vitro cytotoxic effects among parent abietyl alcohol and novel fatty acid ester derivatives against MCF7 and hepatocellular carcinoma cell lines
Synthesis of twelve hitherto unreported esters of abietyl alcohol and screening of these esters against four cancer cell lines including one breast cancer line MCF7 and four hepatocellular carcinoma cell lines (HCC...
Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach
Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified pro...