Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 4
Abstract
Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.
Authors and Affiliations
Liu Chunlei , Min Weihong , Liu Jingsheng , Liu Yan , Ma Jingxi , Li Jingjing
Keywords
Related Articles
- EP ID EP89782
- DOI -
- Views 104
- Downloads 0
In vivo anticoccidial effects of Azadirachta indica and Carica papaya L. with salinomycin drug as a dietary feed supplement in broiler chicks
A total of thirty suspected broiler chicks were screened for coccidiosis, of them 25 chicks were found to be infected with coccidiosis viz. Eimeria tenella (15) Eimeria maxima (5) Eimeria necatrix (6) and Eimeria m...
The hypolipidemic effect of artesunate and ursolic acid in rats
To find the novel hypolipidemic agents, the effects of ursolic acid and artesunate on hyperlipidemia were determined in rats fed a Western-type diet developed a hyperlipidemia. Rats received ursolic acid (50 mg/kg)...
Optimization of enzyme complexes for efficient hydrolysis of corn stover to produce glucose
Hydrolysis of cellulose to glucose is the critical step for transferring the lignocellulose to the industrial chemicals. For improving the conversion rate of cellulose of corn stover to glucose, the cocktail of cel...
Borneol inhibits TRPA1, a proinflammatory and noxious pain-sensing cation channel
Borneol, a natural product isolated from several species of Artemisia, Blumea and Kaempferia, has a widespread use in traditional medicine. TRP ion channels are a class of nonselective cation channel proteins invol...
Effects of rocuronium pretreatment on muscle enzyme levels following succinylcholine
Succinylcholine revolutionized anaesthetic practice by providing intense neuromuscular blockade of very rapid onset and ultrashort duration, thereby greatly easing the maneuver of tracheal intubation. However the w...