Rhodamine-123: A p-glycoprotein marker complex with sodium lauryl sulfate
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 2
Abstract
Aim of this study was to investigate the role of sodium lauryl sulfate (SLS) as P- glycoprotein inhibitor. The everted rat gut sac model was used to study in-vitro mucosal to serosal transport of Rhodamine-123 (Rho-123). Surprisingly, SLS decreases the serosal absorption of Rho-123 at all investigated concentrations. Investigation reveals complex formation between Rhodamine-123 and sodium lauryl sulfate. Interaction profile of SLS & Rho-123 was studied at variable SLS concentrations. The SLS concentration higher than critical micelle concentration (CMC) increases the solubility of Rho-123 but could not help in serosal absorption, on the contrary the absorption of Rho-123 decreased. Rho-123 and SLS form pink color complex at sub-CMC. The SLS concentrations below CMC decrease the solubility of Rho-123. For further studies, Rho-123 & SLS complex was prepared by using solvent evaporation technique and characterized by using differential scanning calorimeter (DSC). Thermal analysis also proved the formation of complex between SLS & Rho-123. The P values were found to be significant (<0.05) except group comprising 0.0001% SLS, and that is because 0.0001% SLS is seems to be very low to affect the solubility or complexation of Rho-123.
Authors and Affiliations
Abdullah M Al-Mohizea , Fahad Ibrahim Al-Jenoobi , Mohd Aftab Alam
Keywords
Related Articles
- EP ID EP100376
- DOI -
- Views 79
- Downloads 0
Immune effects of extractives on bamboo biomass self-plasticization
Bamboo is a fast-growing renewable bioresource. However, bamboo resources are wasted, and bamboo products release toxic gases. Bamboo biomass was therefore extracted and self-plasticized, and the immune effects of...
Haloperidol-induced extra pyramidal symptoms attenuated by imipramine in rats
Effects of administration of imipramine (IMI) are determined on haloperidol-induced extrapyramidal symptoms (EPS). Haloperidol is administered orally at a dose of 0.2 mg/rat/day in rats for a period of 5 weeks, by...
Dimethyl 3, 3’, 4, 4’-tetrahydroxy-δ-truxinate isolated from the leavesof Andrographis lineata.Wall. ex. Nees suppress adipogenesis in 3T3-L1 preadipocytes for type 2 diabetes
The present investigation elucidates the isolation and characterization of bioactive compound from the ethanolic leaf extract of Andrographis lineata (EtALL) which suppress the differentiation of 3T3-L1 adipocytes....
Effect of aqueous extract of Dicranopteris linearis leaves against paracetamol and carbon tetrachloride-induced liver toxicity in rats
The present study aimed to determine the hepatoprotective activity of Dicranopteris linearis L. (family Gleicheniaceae) leaf aqueous extract (DLAE) using two models of liver injury in rats. Rats were divided into t...
Optimization of sustained release matrix tablet of metoprolol succinate using central composite design
The present study was performed to optimize the formulation of metoprolol succinate (MS) sustained release tablets using hydroxypropyl methylcellulose (HPMC) and sodium alginate (SA) as the matrix combination. Afte...