STUDY OF METALLO-Β-LACTAMASE PRODUCTION IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN A TEACHING HOSPITAL OF RURAL GUJARAT - INDIA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2013, Vol 2, Issue 18
Abstract
[b]BACKGROUND[/b]: Pseudomonas aeruginosa exhibits intrinsic resistance to a variety of antimicrobials including beta lactams. Imipenem-resistant Pseudomonas aeruginosa resulting from metallo-β-lactamases production represent a significant and rapidly emerging problem and threat in most part of the world. Detection of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is crucial for the optimal antibiotic treatment of the seriously ill patients. AIM OF THE [b]STUDY[/b]: The study was undertaken to detect Metallo-β-lactamases among clinical isolates of Pseudomonas aeruginosa in the multidisciplinary tertiary care 520-bedded hospital. [b]MATERIAL[/b] [b]AND METHODS[/b]: Two hundred eighty three consecutive clinical isolates of Pseudomonas aeruginosa were subjected to susceptibility testing by disc-diffusion assay and mini API system (bioMerieux-France). Imipenem resistant Pseudomonas aeruginosa isolates were studied for metallo-β-lactamase production by IPM-EDTA-disk synergy test. [b]RESULTS[/b]: Imipenem- resistance by disc diffusion method was found in 35 out of 283 isolates (12.3%) of Pseudomonas aeruginosa. Of the 35 imipenem-resistant isolates 30 (85.7%) were Metallo-beta-lactamases producers with average zone diameter difference of 16.5 mm between imipenem disk and imipenem plus EDTA disk. Of 35 imipenem-resistant isolates, 21(60%) were from ICU patients and 14(40%) were from Wards.[b] CONCLUSION[/b]: IPM-EDTA-disk synergy test can be used as a suitable screening method in the clinical microbiology laboratory. Presence of Metallo-β-lactamase production among imipenem-resistant Pseudomonas aeruginosa was high in our institution which is disturbing and forces us for prompt infection control measures which are well-organized and continued. Combined approaches of rotational antibiotic therapy and training programmes for the health care workers might be beneficial to fight against these multi drug resistant pathogens
Authors and Affiliations
Yagnesh Pandya, Agravat H. H
Keywords
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