The effect of enzyme digestion time on the detection of diatom species
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 3
Abstract
This study is aimed at detecting diatom in lung, liver and kidney tissues using PCR - DHPLC technology after different periods of enzyme digestion to assess the effect of enzyme digestion on the detection of diatom species. Twenty Randomly selected experimental rabbits were drowned at the same place. Their liver, kidney, and lung tissues were removed for sampling. After the extraction of DNA from the samples, amplification was conducted with specific primers of the SSU gene of diatom. Then, an analysis was performed with agarose gel electrophoresis and DHPLC. Within 2 h-8 h, the amount of the diatom species found in the lung gradually increased over time and was statistically significant (P<0.05). After 8 h, with enzyme digestion, the amount of the diatom species found in lung showed no significant increase (P>0.05). However, as for the liver and kidney, within 2h-6h, the amount of the diatom species gradually increased over time and was statistically significant (P<0.05). After 6h, the fig. did not present significant growth (P>0.05). The amount of the diatom species found in the organs after different periods of digestion time had significant differences, which provides a reference for the detection of diatoms and also, has a good application prospect in the forensic identification of drowning.
Authors and Affiliations
Zhengliang Yu , Chao Liu , Huijun Wang , Ling Chen1 , Sunlin Hu , Jian Zhao , Huipin Wang , Yu Zhou , Weibing Xie
Keywords
Related Articles
- EP ID EP115864
- DOI -
- Views 73
- Downloads 0
Salmon calcitonin in the treatment of elderly women with type 2 diabetes complicated with osteoporosis
To explore the reasonable treatment scheme of salmon calciteonin in the treatment of elderly women with type 2 diabetes complicated with osteoporosis, patients were randomly divided into Group A, B and C, and they...
Bioequivalence study of two oral formulations of clarithromycin in human male subjects
To assess the bioequivalence of tablets formulations of Clarithromycin 500mg each of test and reference products. A single post oral dose of each formulation was given to 14 male healthy volunteers. The study was conduct...
Evaluation of antimicrobial activity of extracts of in vivo and in vitro grown Vinca rosea L. (Catharanthus roseus) against pathogens
The antimicrobial activity of Vinca rosea was evaluated against pathogenic bacterial strains (Bacillus subtilis, B. licheniformis and Azotobacter sp.) and fungal strains (Asprgillus niger, Alternaria solani and Rhi...
Effects of medetomidine on serum glucose in cattle calves
An experimental study was carried out to compare physiological effects (serum glucose level) of medetomidine in Red Sindhi cattle calves at three different doses i.e. 8, 10 and 12µg/kg body weight intravenously. Me...
The preparation of the sustained release metformin hydrochloride microcapsules by the Wurster fluidized bed
The main objective of this study was to prepare sustained release metformin hydrochloride microcapsules by the Wurster fluidized bed and to obtain the optimized coating process and formulation. Fine microcapsules w...