The influence of joint application of arsenic trioxide and daunorubicin on primary acute promyelocytic leukaemia cells and apoptosis and blood coagulation of cell strain
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 3
Abstract
This test cultivated three groups of acute promyelocytic leukemia (APL) and NB4 cells in liquid in vitro, processed them with arsenic trioxide (ATO), daunorubicin (DNR), ATO+DNR respectively, and then set up blank control group. Apoptosis of cells in each group was observed using flow cytometry, procoagulant activity of APL and NB4 cells in each group was detected with recalcification time, and expressions of tissue factor (TF), thrombomodulin and annexin II of NB4 cells in each group were measured using ELISA method. The results showed that the apoptosis rate increased 4-8 times compared with blank control group after processing APL and NB4 cells with ATO and DNR; procoagulant activity decreased obviously; and expression of TF and annexin II of NB4 cells reduced significantly (P<0.05). We concluded that combination of ATO and DNR could promote APL and NB4 cell apoptosis effectively without aggravating blood coagulation disorders, which might improve coagulation function of APL by inhibiting coagulation and hyperfibrinolysis through reducing expression of TF and annexin II. This drug combination may be a safe and effective method in the treatment of APL of primary high white blood cells type.
Authors and Affiliations
Xiaojuan Zhang , Na Qin , Xinghua Chen , Shuxia Guo
Keywords
Related Articles
- EP ID EP89537
- DOI -
- Views 91
- Downloads 0
In vivo anticoccidial effects of Azadirachta indica and Carica papaya L. with salinomycin drug as a dietary feed supplement in broiler chicks
A total of thirty suspected broiler chicks were screened for coccidiosis, of them 25 chicks were found to be infected with coccidiosis viz. Eimeria tenella (15) Eimeria maxima (5) Eimeria necatrix (6) and Eimeria m...
The characteristics of acromegalic patients with hyperprolactinemia and the differences with hyperprolactinemia patients
A substantial proportion of acromegalic patients have co-existent hyperprolactinaemia. To compare this group of population (AC+HPRL) to those of patients with merely hyperprolactinemia (HPRL), a retrospective analy...
Determination of total polyphenolic compounds and flavonoids in Juglans regia leaves
Juglans regia leaves have been widely used in traditional medicines because of its antimicrobial, antihelmintic, astringent, keratolytic, antidiarrhoeal, hypoglycaemic, depurative, tonic, carminative activity. Tota...
Antioxidant study of flavonoid derivatives from the aerial parts of Rhus natalensis growing in Saudi Arabia
Phytochemical study of the CH2Cl2 soluble fraction of the aerial parts of R. natalensis resulted in the isolation and identification of six flavonoid derivatives, -amyrin and -sitosterol glucoside (daucosterol)....
Detection of Mycobacterium tuberculosis and Mycobacterium bovis from human sputum samples through multiplex PCR
Tuberculosis (TB) has a long history and being present even before the start of recording history. It has left detrimental effects on all aspect of the life and geared the developments in the science of health. TB...